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pLY57_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2-TRAC-RHA(3166mut)

Plasmid

#192188

Purpose

CD22 CAR AAV vector PRODH2 KI (pLY057)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

CD22 CAR AAV vector PRODH2 KI (pLY057) (CD22 Synthetic)

Use

AAV; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLY59_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2(stop)-TRAC-RHA(3166mut)

Plasmid

#192189

Purpose

CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059) (CD22 Synthetic)

Use

AAV; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-748_HA-GD2-28z_CAR_BATF

Plasmid

#207488

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-755_HA-GD2-28z_CAR_RFP-JUN

Plasmid

#207494

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-JUN, HA-GD2-28z_CAR (JUN Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-804_HA-GD2-28z_CAR_BATF-TFAP4

Plasmid

#207491

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF-TFAP4, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-805_HA-GD2-28z_CAR_BATF-RFP

Plasmid

#207492

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF-RFP, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-28z

Plasmid

#135991

Purpose

Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

June 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.28H.28TM.BB.3z

Plasmid

#200679

Purpose

Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.8H.8TM.BB.3z

Plasmid

#200671

Purpose

Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD8TM-41BB-CD3z-P2A-EGFP (CD8A, TNFRSF9, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-CD3z

Plasmid

#135993

Purpose

Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-3z-P2A-EGFP (CD247, CD28 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.28H.28TM.BB.28.3z

Plasmid

#200681

Purpose

Modular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)

Plasmid

#192190

Purpose

HER2 CAR AAV vector PRODH2 KI (pLY080b)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

HER2 CAR AAV vector PRODH2 KI (pLY080b) (ERBB2 Synthetic)

Use

AAV; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLY095-EFS-ERBB2 HER2-Blast-WPRE

Plasmid

#192196

Purpose

Lenti-HER2-OE-Blast

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-HER2-OE-Blast (ERBB2 Synthetic)

Use

Lentiviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINO_NbH11-H4

Plasmid

#155364

Purpose

Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

Depositor

Ray Owens

Article

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

H11-H4

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pOPINO_NbH11-D4

Plasmid

#155363

Purpose

Expresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

Depositor

Ray Owens

Article

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

Nb H11-D4

Use

Tags

His6

Expression

Mutation

Promoter

T7

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pOPINO_H3

Plasmid

#171927

Purpose

Bacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Article

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_H3

Use

Tags

His6

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pOPINO_F2

Plasmid

#171926

Purpose

Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Article

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_F2

Use

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19-HDRT-CD3z-truncCAR(anti-CD19)

Plasmid

#215758

Purpose

HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2

Depositor

Dimitrios Wagner

Article

Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

Insert

CD247-truncCD19CAR (CD247, CD19 Human)

Use

Tags

IgG1 CH3

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-BBz

Plasmid

#135992

Purpose

Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1A-CD19.28H.28TM.28.BB.3z

Plasmid

#200680

Purpose

Modular CD28hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge