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Plasmid#35507PurposeAAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123APromoterEf1aAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSN840
Plasmid#184832PurposeExpresses human KIF5A(∆exon27) and human KLC1 in insect cellsDepositorUseCre/LoxTagsHis-FLAG and mScarlet-2xStrepIIExpressionInsectMutation∆exon27 form of human KIF5APromoterp10 and polyhedrinAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40
Plasmid#100855PurposeAAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoterDepositorInsertjRGECO1b
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)
Plasmid#154840PurposeInsertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.DepositorInserthomology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
UseCRISPR and Cre/LoxTagsExpressionWormMutationHYGR∆ encodes aa 1-226PromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDragon-Ctgf
Plasmid#155015PurposeFor Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.DepositorInsertCcn2 (Ccn2 Mouse)
UseMouse TargetingTagsExpressionMutationM24T (please see depositor comments)PromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OTmut
Plasmid#185389PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSECC-sgTom
Plasmid#138661PurposeExpresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinaseDepositorInsertsgTomato
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB-MultiplexedReporter-MOR170-1
Plasmid#129461PurposepiggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter geneDepositorInsertMOR170-1 (Olfr895 Mouse)
UseSynthetic BiologyTagsLUCY tag, Rho tagExpressionMammalianMutationPromoterTRE (Tet-On)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2122_pCAG-PV1-GAI-L1-Bxb1-469C-NLS-BGHpA
Plasmid#108816PurposeExpresses aa469 to C-terminus of Bxb1 integrase fused to GAI CIDDepositorInsertBxb1 integrase fragment aa469 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-OFF Kir2.1-2A-GFP
Plasmid#161580PurposeExpress Kir2.1 in cells not expressing Cre.DepositorInsertKir2.1-2A-GFP (Kcnj2 Mouse)
UseAAVTagsExpressionMutationNAPromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW1599_pCAG-PV1-PhiC31-N233-L1-ABI-NLS-BGHpA
Plasmid#108797PurposeExpresses N-terminus to aa233 of PhiC31 integrase fused to ABI CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa233
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1604_pCAG-PV1-PYL-L1-PhiC31-234C-BGHpA
Plasmid#108798PurposeExpresses aa234 to C-terminus of PhiC31 integrase fused to PYL CIDDepositorInsertPhiC31 integrase fragment aa234 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-DIO-TRPV1-P2A-mCherry
Plasmid#200831PurposeIn the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherryDepositorInsertTRPV1-P2A-mCherry (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-EYFP-P2A-cMaf
Plasmid#98174PurposeRetroviral vector encoding Cre-dependent expression of EYFP-P2A-MafDepositorInsertcMaf (codon optimzed) (Maf Mouse)
UseRetroviralTagsExpressionMutationCodon optimizedPromoterCAGAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-flex-GFP-shRNA-GluN1
Plasmid#182502PurposeExpresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoterDepositorInsertpan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
UseAAV, Cre/Lox, and RNAiTagsExpressionMammalianMutationPromoterfragment of histidine decarboxylase gene promoter…Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1846
Plasmid#154326PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::GFP^SEC^AID*::3xFLAG
UseCRISPR and Cre/LoxTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1582
Plasmid#154312PurposemNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertmNeonGreen^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Th-tdTomato
Plasmid#159460PurposeTargeting vector to TH gene locus for induction of P2A-fused tdTomato, with floxed PGK-puroR drug resistant cassetteDepositorInsertP2A-tdTomato
UseCre/LoxTagsP2AExpressionMammalianMutationPromoterEndogenous THAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HDC-DIO-GFP-shRNA.scramble
Plasmid#184331PurposeExpresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expressionDepositorInserthdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
UseAAV, Cre/Lox, Mouse Targeting, and RNAiTagsExpressionMammalianMutationPromoterhistidine decarboxylase promoter fragment that g…Available sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.NES-jRGECO1b.WPRE.SV40
Plasmid#100856PurposeAAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorInsertjRGECO1b
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only