-
Plasmid#31139DepositorInsertADAM7 (ADAM7 Human)
UseTagsFLAGExpressionMammalianMutationwtPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb7-PIR-SH2
Plasmid#46443DepositorInsertGrb7 (GRB7 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 334-532PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-PRAS40
Plasmid#53599Purposelentiviral expression of constitutively active PRAS40 and EGFPDepositorInsertPRAS40 (AKT1S1 Human)
UseLentiviralTagsIRES-EGFP and mycExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat2-SH2
Plasmid#46512DepositorInsertStat2 (STAT2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 566-681PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
J23112-mRFP1-331Bb
Plasmid#78278PurposemRFP1 behind constitutive promoter J23112 in pSEVA331Bb backboneDepositorInsertJ23112-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23103-mRFP1-331Bb
Plasmid#78273PurposemRFP1 behind constitutive promoter J23103 in pSEVA331Bb backboneDepositorInsertJ23103-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23105-mRFP1-331Bb
Plasmid#78275PurposemRFP1 behind constitutive promoter J23105 in pSEVA331Bb backboneDepositorInsertJ23105-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IRES-miR34a-P2
Plasmid#64786PurposeLuciferase expression driven by miR-34a promoter P2DepositorInsertmiR-34a promoter P2 (MIR34A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-195-5p
Plasmid#103312PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-195-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-195-5p target (MIR195 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX HSH2-SH2
Plasmid#46446DepositorInsertHSH2 (HSH2D Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-134PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
J23101-mRFP1-331Bb
Plasmid#78272PurposemRFP1 behind constitutive promoter J23101 in pSEVA331Bb backboneDepositorInsertJ23101-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX ShcC-SH2
Plasmid#46487DepositorInsertShcC (SHC3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 485-594PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-15b-5p
Plasmid#103269PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-15b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-15b-5p target (MIR15B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs2-SH2
Plasmid#46505DepositorInsertSocs2 (SOCS2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 28-162PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTet02
Plasmid#78284PurposemRFP1 expressed behind an ATc-inducible constructDepositorInsertTet inducible mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
D-raf R217L
Plasmid#39762DepositorInsertd-raf (Raf Fly)
UseTagsExpressionInsectMutationR217LPromoterAvailable sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
Rescue Septin 11 mRNA
Plasmid#122025PurposeRescue mRNA for Septin 11 Knock Down with sh2 shRNA. Splice variant II (EU711415) with 5 silent mutations at SEPT11 sh2 shRNA target site.DepositorInsertSEPT11 (Sept11 Rat)
UseTagsExpressionMammalianMutationSilent mutations are in 5 consecutive codons (CUG…PromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-17-5p
Plasmid#103274PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-17-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-17-5p target (MIR17 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4B (205-224) (pGEX2T(TEV))
Plasmid#80634PurposeResidues 205-224 of CHMP4B, Bacterial Expression Vector; TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201DepositorInsertCHMP4B (CHMP4B Human)
UseTagsGSTExpressionBacterialMutationResidues 205-224PromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-285/-192
Plasmid#31053DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutation-285 to -1 of P2 HNF4A promoter with mutation -19…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-130a-3p
Plasmid#103216PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-130a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-130a-3p target (MIR130A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-28-3p
Plasmid#103385PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-28-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-28-3p target (MIR28 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-510-3p
Plasmid#103592PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-510-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-510-3p target (MIR510 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-339-3p
Plasmid#103452PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-339-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-339-3p target (MIR338 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-376c-5p
Plasmid#103500PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-376c-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-376c-5p target (MIR376C Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-340-5p
Plasmid#103459PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-340-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-340-5p target (MIR340 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7b-5p
Plasmid#103148PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7b-5p target (MIRLET7B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30e-5p
Plasmid#103419PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30e-5p target (MIR30E Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR3c-K717M
Plasmid#45715DepositorInsertFGFR3 IIIc (FGFR3 Human)
UseRetroviralTagsExpressionMammalianMutationK717MPromoterAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR2b-S320C
Plasmid#45702DepositorInsertFGFR2 IIIb (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationS320CPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-615-5p
Plasmid#103675PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-615-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-615-5p target (MIR615 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-584-5p
Plasmid#103665PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-584-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-584-5p target (MIR584 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7g-5p
Plasmid#103158PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7g-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7g-5p target (MIRLET7G Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTBL649 CHYRON2 integration construct
Plasmid#126446PurposeTo integrate the CHYRON2 locus at AAVS1 in human cells.DepositorInsertspU6-CHYRON2 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX p55g(NC)-SH2
Plasmid#46462DepositorInsertp55g(NC) (PIK3R3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 56-461PromoterTacAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH2D4B-SH2
Plasmid#46482DepositorInsertSH2D4B (Sh2d4b Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 311-431PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Dapp1-SH2
Plasmid#46422DepositorInsertDapp1 (DAPP1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 25-151PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Sck-SH2
Plasmid#46478DepositorInsertSck/ShcB (Shc2 Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 464-573PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nsp3-SH2
Plasmid#46461DepositorInsertNsp3 (SH2D3C Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 212-330PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH2D5-SH2
Plasmid#46483DepositorInsertSH2D5 (Sh2d5 Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 290-399PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-125a-5p
Plasmid#103188PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-125a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-125a-5p target (MIR125A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Mist-SH2
Plasmid#46454DepositorInsertMist (Clnk Mouse)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 300-435PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH2-B-SH2 RA
Plasmid#46480DepositorInsertSH2-B RK (SH2B1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 515-639; R555A mutation in t…PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-876-3p
Plasmid#103742PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-876-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-876-3p target (MIR876 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-377-3p
Plasmid#103501PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-377-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-377-3p target (MIR376C Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-34a-5p
Plasmid#103465PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-34a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-34a-5p target (MIR34A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-377-5p
Plasmid#103502PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-377-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-377-5p target (MIR377 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-203a-3p
Plasmid#103336PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-203a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-203a-3p target (MIR203A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-301a-3p
Plasmid#103396PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-301a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-301a-3p target (MIR301A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-505-3p
Plasmid#103583PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-505-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-505-3p target (MIR505 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only