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Showing: 6241 - 6280 of 7539 results

  5. pDR111_mannose_Klf4Gata-3

    Plasmid
    #188401
    Purpose
    Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Inserts
    Klf4 (Klf4 Mouse)
    Gata-3 (Gata3 Mouse)
    Use
    Tags
    PhoD secretion peptide
    Expression
    Bacterial
    Mutation
    See Depositor Comments Below
    Promoter
    D-mannose
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188401-plasmid-map-sequence-id-370467

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  9. EFSp-GFP-YAP (5SA)

    Plasmid
    #174170
    Purpose
    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.
    Depositor
    Rod Bremner
    Article
    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610
    Insert
    YAP (YAP1 Human)
    Use
    Lentiviral
    Tags
    3x FLAG tag
    Expression
    Mammalian
    Mutation
    Five LATS phosphorylation sites (S61, S109, S127,…
    Promoter
    EFS
    Available since
    Sept. 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    174170-plasmid-map-sequence-id-343725

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  10. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71685-plasmid-map-sequence-id-322543

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  11. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71684-plasmid-map-sequence-id-129672

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  12. PGKp-GFP-YAP (5SA)

    Plasmid
    #174174
    Purpose
    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.
    Depositor
    Rod Bremner
    Article
    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610
    Insert
    YAP (YAP1 Human)
    Use
    Lentiviral
    Tags
    3x FLAG tag
    Expression
    Mammalian
    Mutation
    Five LATS phosphorylation sites (S61, S109, S127,…
    Promoter
    PGK
    Available since
    Aug. 19, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    174174-plasmid-map-sequence-id-340277

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  13. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  26. pBoBi-hAP1-C-LAMP2 TM

    Plasmid
    #183645
    Purpose
    Lentiviral expression of a mutant of ATP6AP1 with its PEN2-interacting domain replaced with the transmembrane (TM) domain of LAMP2.
    Depositor
    Sheng-cai Lin
    Article
    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:
    Insert
    ATP6AP1 and LAMP2 (ATP6AP1, LAMP2 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    ATP6AP1 transmembrane domain replaced by the tran…
    Promoter
    CMV
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183645-plasmid-map-sequence-id-363129

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  27. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid
    #92139
    Purpose
    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92139-plasmid-map-sequence-id-349059

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  33. pUCmini-iCAP-AAV.CAP-B10

    Plasmid
    #175004
    Purpose
    non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B10 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175004-plasmid-map-sequence-id-347131

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  35. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid
    #85452
    Purpose
    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
    Depositor
    Aviv Regev
    Article
    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do
    Insert
    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    85452-plasmid-map-sequence-id-173969

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  37. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

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  39. HR_G3BP1-V5-APEX2-GFP

    Plasmid
    #105284
    Purpose
    homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus
    Depositor
    Eugene Yeo
    Article
    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do
    Insert
    G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)
    Use
    Human homologous recombination donor
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105284-plasmid-map-sequence-id-229022

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Showing: 6241 - 6280 of 7539 results