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pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100850

Purpose

AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1b

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-tTA

Plasmid

#127091

Purpose

An AAV genome with Cre-dependent expression of tTA from the CAG promoter

Depositor

Viviana Gradinaru

Article

Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

Insert

tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

*(see below)

Promoter

CAG

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pFrt-invCAG-ires-Luc

Plasmid

#63576

Purpose

Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.

Depositor

Ivo Huijbers

Article

Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

Insert

Stop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG

Use

Cre/Lox; Frt/flpe recombination mediated insertion

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA

Plasmid

#108789

Purpose

Expresses N-terminus to aa233 of PhiC31 integrase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment N-terminus to aa233

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpA

Plasmid

#108790

Purpose

Expresses aa234 to C-terminus of PhiC31 integrase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment aa234 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

Plasmid

#98927

Purpose

Cre dependent AAV expression of GFP spaghetti monster myc construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has Service

AAV1

Insert

GFP-smFP-myc

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OT1.0

Plasmid

#185387

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-CAG::Mitbow

Plasmid

#158994

Purpose

Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Mitbow

Use

Tags

The default expressed EBFP2 is fused to H2B; oth…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

Plasmid

#190236

Purpose

AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

F2A, LDLR C-terminal sequence, mRFP1, myristoylat…

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CALID

Plasmid

#169708

Purpose

Cre-activated light-inducible Dre recombinase

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-SomArchon

Plasmid

#126943

Purpose

Cre-dependent expression of SomArchon under CAG promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#108770

Purpose

Expresses aa397 to C-terminus of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa397 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDragon-p21

Plasmid

#155016

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activity

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

Cdkn1a (Cdkn1a Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

Plasmid

#205098

Purpose

Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

Inserts

mNeonGreen
eKaz luciferase
Volvox Channelrhodopsin 1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA

Plasmid

#108833

Purpose

Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa28 to aa396

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS and GAI

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-DIO-intron336-dLUC

Plasmid

#110056

Purpose

Cry1 transcription luciferase reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and luciferase (Cry1 Mouse)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Cry1

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

Plasmid

#35509

Purpose

AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV5 and AAV9

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_eGFP::Dpp

Plasmid

#163702

Purpose

GFP-tagged version of Dpp (Decapentaplegic) under control of UAS and LexO enhancers

Depositor

Markus Affolter

Article

Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

Insert

decapentaplegic (dpp Fly)

Use

Cre/Lox

Tags

Tagged with eGFP

Expression

Insect

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-EYFP-P2A-Mafb

Plasmid

#98175

Purpose

Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Mafb

Depositor

Oscar Marin

Article

Mi et al Science. 2018 Feb 22. pii: science.aar68

Insert

Mafb (Mafb Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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AAV8-hSyn-flex-miR30-eGFP-shDyn

Plasmid

#132716

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Pdyn gene, which encodes dynorphin

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shDyn (Pdyn Rat)

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin 1

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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