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Plasmid#13822PurposeMammalian expression of human histone deacetylase 5 with flag tagDepositorInsertHDAC5 (HDAC5 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
h-alpha-ENaC
Plasmid#83430Purposefull length human alpha ENaC with Flag tagDepositorInserthuman alpha ENaC (SCNN1A Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
V5-GFP-TurboID-KDEL_pLX208
Plasmid#202020Purposeexpresses GFP-TurboID in the mammalian ER lumen, lentiviral vectorDepositorInsertV5-TurboID-KDEL
UseLentiviralTagsGFP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG
Plasmid#46956PurposePlasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.DepositorInsertFLAG tag
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-ER-LAR-GECO1
Plasmid#61244PurposeExpresses LAR-GECO1 in the endoplasmic reticulum in mammalian cellsDepositorInsertLAR-GECO1
UseTagsER-retention sequence: KDEL and ER-targeting sequ…ExpressionMammalianMutationSubstitutions relative to R-GECO1: V51W/I113V/N35…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GFP
Plasmid#175757PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)DepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3z/601
Plasmid#26656DepositorInsert
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v2-10 (fl)
Plasmid#137824Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v2-10 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull length and mutations A282T, T483A, N535D, S5…PromoterPGKAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3
Plasmid#90087PurposeExpresses wild type caspase 3 in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationWild typePromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Puro DEST (670-1)
Plasmid#17293Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->mCherry_PGK-->BFP
Plasmid#162231PurposeLentiviral vector for the expression of TRE3GS-->mCherry_PGK-->BFP in mammalian cellsDepositorInsertmCherry and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagB 99L
Plasmid#19303DepositorInsertRas-related GTP binding B (RRAGB Human)
UseTagsGST and HAExpressionMammalianMutationQ99LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
RA-Sec61β
Plasmid#153978PurposeDimerization dependent fluorescent protein RA domain fused to ER protein Sec61βDepositorInsertSec61 Transolocon Subunit Beta (SEC61B Human)
UseTagspAcGFP-C1ExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Tet1CD
Plasmid#84475PurposeLentiviral construct to express dCas9 fused with the catalytic domain of Tet1DepositorInsertdCas9-Tet1CD
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MMLVgag-3NES-Cre
Plasmid#207252PurposeGeneration of CreVLPs when used in conjunction with MMLV gag-pol during productionDepositorInsertMMLVgag-3NES-Cre
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-TFE3
Plasmid#38120DepositorInsertTFE3 (TFE3 Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
anxA2-GFP
Plasmid#107196PurposeExpresses human annexin A2-GFP fusion protein for live cell imagingDepositorInsertannexin A2 (ANXA2 Human)
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationAla residue 65 in human sequence replaced by Glu …PromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI3M-TBD
Plasmid#113077PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI3M-TBD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCherry-(F30-2xPepper)10
Plasmid#129401PurposemCherry mRNA reporter tagged with (F30-2xPepper)10DepositorInsertmCherry-(F30-2xPepper)10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoTPNOX
Plasmid#87854PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertmitoTPNOX
UseTagsC-terminal FLAG tag with a linker and mitochondri…ExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330S-2
Plasmid#58778PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 human p27
Plasmid#14049DepositorInsertp27 (CDKN1B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
PEmax-mRNA
Plasmid#204472Purposeplasmid for PEmax mRNA IVTDepositorInsertPrime editor
UseCRISPRTagsnoExpressionMammalianMutationPromoterT7Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shp53/GFP4
Plasmid#20208DepositorInsertshp53/GFP4
UseTransposonTagsExpressionMutationPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8r
Plasmid#208318PurposeOverxpression MBP-TadA8rDepositorInsertTadA8r
UseTagsExpressionBacterialMutationmutations in TadA are described in the paperPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP670
Plasmid#136571PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertH2B-emiRFP670
UseTagsemiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1
Plasmid#27099DepositorInsertα-Tubulin K40 acetyltransferase1 (ATAT1 Human)
UseTagsGFP, S-tag, and TEV siteExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.eGFP.2A.P301L-Tau
Plasmid#140425PurposeAAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTauDepositorInserteGFP-2A-Tau(P301L) (MAPT Human, Synthetic)
UseAAVTagseGFPExpressionMutationchanged Proline 301 to LeucinePromoterCBAAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42(wt)
Plasmid#12599DepositorInsertEGFP-Cdc42 (WT) (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLAI-Env
Plasmid#133996PurposeEnv gene from HIV-1 LAI in pcDNA3.1 expression vectorDepositorInsertEnvelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only