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pcDNA3.1-cox8-iPASnFR-H-mScarlet3-3xFLAG

Plasmid

#225239

Purpose

Mammalian expression of iPASnFR-H in mitochondria

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Use

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAC154-dual-dCas9VP160-sgExpression

Plasmid

#48240

Purpose

Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP160

Expression

Mammalian

Mutation

D10A H840A

Promoter

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-T7 RNA POL

Plasmid

#138352

Purpose

Expressing T7 RNA Polymerase in mammalian cells

Depositor

Baisong Lu

Insert

T7 RNA POL

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRi (Gen2)

Plasmid

#73498

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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GST-RH▲-MNase

Plasmid

#136292

Purpose

Expression of catalytic inactive RNase H fused to micrococcal nuclease

Depositor

Kavitha Sarma

Article

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

RNase HI-D10R-E48R and MNase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-Sumo1 WT

Plasmid

#48966

Purpose

Expresses HA-Sumo1 WT in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO1 (SUMO1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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spNW15

Plasmid

#173483

Purpose

Expresses spNW15 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW15

Use

Tags

Histag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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GST-MNase

Plasmid

#136291

Purpose

Expresses Micrococcal nuclease

Depositor

Kavitha Sarma

Article

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

MNase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs GFP-LC3-RFP

Plasmid

#117413

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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gag-PE

Plasmid

#211371

Purpose

Packaging plasmid for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-PE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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