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Plasmid#137115PurposeProtease Switch Clone ID: 1-A9DepositorInsertAI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV
Plasmid#137116PurposeProtease Switch Clone ID: 1-C9DepositorInsertAI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1_AscI_V5-gateway_Bsu36I
Plasmid#128467Purposedestination vector with N-terminal V5 tagDepositorInsertdestination vector with N-terminal V5 tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S2_AscI_HA-gateway_Bsu36I
Plasmid#128468Purposedestination vector with N-terminal HA tagDepositorInsertdestination vector with N-terminal HA tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
C-term png in pGEX4
Plasmid#112975PurposeFor protein expression and purification of the C-term of Drosophila pngDepositorInsertpng (png Fly)
UseTagsExpressionBacterialMutationwild-type C terminusPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS316Nab3-11
Plasmid#110535PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationtwo missense mutations creating Nab3-11PromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3-11
Plasmid#110534PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationtwo missense mutations creating Nab3-11PromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6637
Plasmid#106164PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistanceDepositorInsertunknown
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_S1966C
Plasmid#98681PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966CDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationS1966CPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-RORbshRNA2
Plasmid#73984PurposeRORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse RORb gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-RORbshRNA1
Plasmid#73985PurposeRORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse RORb gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-RORbshRNA2
Plasmid#73986PurposeRORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse RORb gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-Otx2shRNA2
Plasmid#73980PurposeOtx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-RORbshRNA1
Plasmid#73983PurposeRORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse RORb gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
Plasmid#68276PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLSDepositorInsertSte7DS NLS(4E) mCherry
UseTagsmCherryExpressionYeastMutationPromoterRPS2Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVK_FG
Plasmid#66070PurposeMoClo Destination Vector: (based on pSB1K3) [F:LacZa:G]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVK_GH
Plasmid#66071PurposeMoClo Destination Vector: (based on pSB1K3) [G:LacZa:H]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pED55 pRS306 pRPS2-SteND-NLS-mCherry
Plasmid#68277PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutationDepositorInsertSte7ND NLS mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry
Plasmid#68275PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLSDepositorInsertSte7DS NLS(4A) mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBacPak-HA-CG9866-1-200
Plasmid#52291PurposeExpresses CG9866 residues 1-200DepositorInsertAtaxin-7 (CG9866 Fly)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only