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Plasmid#216832Purposecardiomyocytes-specific plasmid, expressing Gfp and the coding sequence of mouse PalmdDepositorInsertPalmd (mus musculus) (Palmd Mouse)
UseAAVTagsGFPExpressionMutationPromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cTNT-NEXN-GFP
Plasmid#216835Purposecardiomyocytes-specific plasmid, expressing the coding sequence of mouse Nexn and GFP tagDepositorInsertNexn (mus musculus) (Nexn Mouse)
UseAAVTagsGFPExpressionMutationPromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMeCP2Y pc644
Plasmid#167565PurposeThe complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)DepositorInsertMethyl-CpG binding protein 2 (Mecp2 Rat)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Mm_Plk5(300-595) PBD domain
Plasmid#136316PurposeMammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFPDepositorInsertPLK5 (Plk5 Mouse)
UseTagsExpressionMammalianMutationfragment 300-595PromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Mm_MyoGEF(WT)
Plasmid#136327PurposeMouse MyoGEF-WT in Gateway pENTRDepositorInsertPLEKHG6 (Plekhg6 Mouse)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
289aa Met133, 138, 186Ileu ELL2
Plasmid#127272PurposeExpresses 289aa human ELL2 with Met133, 138, 186IleuDepositorInsertELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
UseTagsExpressionMammalianMutationMet133, 138, 186 Ileu, cut with XbaI blunt fillinPromoterpEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
frameshift ELL2
Plasmid#127257PurposeFor in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.DepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationXhoI site between the 1st &2nd Met was filled…PromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
564aa ELL2 bluescript
Plasmid#127267PurposeFor in vitro translation of human ELL2 lacking COOH regionDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationAddgene Plasmid 127256--SphI site filled in, then…PromoterT7Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cx32R220stop
Plasmid#78151PurposeMammalian Expression of the first 219 amino acids of Connexin 32 and EGFP from a bicistronic mRNADepositorInsertConnexin 32 (GJB1 Human)
UseTagsEGFPExpressionMammalianMutationOnly contains first 219 residuesPromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCherry.90 alpha
Plasmid#108222PurposeMammalian expression vector for mCherry fusion to human Hsp90 alphaDepositorInsertmCherry-Hsp90a (HSP90AA1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDyn1
Plasmid#64067PurposeHuman cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).DepositorInsertDYNC1H1 (DYNC1H1 Human)
UseCre/LoxTagsHis-ZZ-TCS-SNAPfExpressionInsectMutationCodon optimized for Sf9PromoterAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-BS-AtMIR390a-B/c
Plasmid#199560PurposePlant expression vector (2x35S) for direct cloning of amiRNAs into Arabidopsis thaliana MIR390a precursor including only the basal stemDepositorInsertBasal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
UseRNAiTagsExpressionMutationPromoter2x35SAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCS-3_Endofin_LgBiT
Plasmid#182574PurposeThis plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.DepositorInsertzinc finger FYVE-type (ZFYVE16 Human)
UseTagsLgBiT (Large BiT)ExpressionMammalianMutationFYVE domain Q739-K806PromoterHerpes simplex virus (HSV)Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
nLuc-GLP-1R-His-FLAG
Plasmid#124831PurposeExpresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cellsDepositorInsertGLP1R (GLP1R Human, Synthetic)
UseLuciferaseTagsFLAG, His6, and NanoLucExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-CAG-hMGT-Ef1a-puro
Plasmid#172395PurposeLentiviral expression vector for human direct cardiac reprogrammingDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424TagsExpressionMutationThe conserved GDD in the NS5 polymerase gene muta…PromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-UBCp-hMGT-Ef1a-puro
Plasmid#172396PurposeLentiviral expression vector for human direct cardiac reprogrammingDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUW-SOK
Plasmid#20327DepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAD29_hIRG1_4-461_pvp008
Plasmid#124843PurposeExpresses human CAD (Irg1) in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-3 and 462-end.PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-3xMBT
Plasmid#46987PurposeInducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
UseTagsGST and PreScission cleavage siteExpressionBacterialMutationcontains amino acids 190–530 of accession NP_0562…PromotertacAvailable sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only