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Plasmid#213619PurposeBacterial expression of SUMO-tagged rat syt1 (96-421)DepositorInsertsyt1 (96-421) (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB - 4A
Plasmid#213625PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB - 4ADepositorInsertsyt7 C2AB - 4A (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationF167A, F229A, I298A, L361APromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2A-2A 2B
Plasmid#213626PurposeBacterial expression of SUMO-tagged mouse syt7 C2A-2A 2BDepositorInsertsyt7 C2A-2A 2B (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationF167A, F229A, R363HPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB-2A
Plasmid#213627PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB-2ADepositorInsertsyt7 C2AB-2A (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationI298, L361APromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-cpHaloTag141/145
Plasmid#194660PurposeProtein production of circular permuted HaloTag (cpHaloTag141/145) in bacteriaDepositorInsertcpHaloTag141/145
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-cpHaloTag154/156
Plasmid#194661PurposeProtein production of circular permuted HaloTag (cpHaloTag154/156) in bacteriaDepositorInsertcpHaloTag154/156
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM43 p62 dUBA (1-389)
Plasmid#210426PurposeBacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage siteDepositorInsertp62 FL dUBA (1-389) (NUP62 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)
Plasmid#205049PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)
UseTagsMGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L1
Plasmid#205050PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L1
UseTagsGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L2
Plasmid#205051PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L2
UseTagsGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L3
Plasmid#205052PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L3
UseTagsGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-tTALE-mTurquoise2-15b
Plasmid#206871PurposeExpress tTALE-mTurquoise2DepositorInsert7-mer tTALE-mTurquoise2
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-tTALE-mNeonGreen-15b
Plasmid#206870PurposeExpress tTALE-mNeonGreenDepositorInsert7-mer tTALE-mNeonGreen
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-tTALE-mKO2-15b
Plasmid#206869PurposeExpress tTALE-mKO2DepositorInsert7-mer tTALE-mKO2
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo-MCS
Plasmid#212725PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-MCS
Plasmid#212724PurposeBacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-MCS-His6
Plasmid#212723PurposeBacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo-GFP
Plasmid#212720PurposeBacterial expression of His6-CUbo-GFPDepositorInsertGFP
UseTags6xHis-CUboExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24-95~236
Plasmid#208634Purposefor E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236DepositorInserthuman RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationonly aa 95~236, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-mEGFP(A206K)-TwStrep
Plasmid#208622Purposefor E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) cloneDepositorInsertmEGFP(A206K)
UseTags6xHis and Twin StrepExpressionBacterialMutation(compared with pEGFP-C1, protein level) A206KPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only