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Plasmid#191800PurposeFull length wt AtPCO3 with Nt-His tagDepositorInsertAtPCO3
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28 sf:em-blue-shift.2
Plasmid#191919PurposeEncodes a GFP design, sf:em-blue-shift.2DepositorInsertsf:em-blue-shift.2
UseTagsHis TagExpressionBacterialMutationT65S S72A T108I Y145F V150IPromoterT7Available sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)-MutTER
Plasmid#178035PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deleted and Changed T(8…PromoterT7LacIAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP2-E67A_rpoA-U
Plasmid#190959PurposeTest the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
UseTagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-A66S_rpoA-C
Plasmid#190968PurposeTest the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP2-A66S_rpoA-U
Plasmid#190957PurposeTest the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
UseTagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3-A66S_rpoA-U
Plasmid#190961PurposeTest the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
UseTagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3-E67A_rpoA-U
Plasmid#190963PurposeTest the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
UseTagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-E67A_rpoA-C
Plasmid#190972PurposeTest the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Arabidopsis thaliana Atm3 pET21a+
Plasmid#172321PurposeExpresses AtAtm3 in E.coliDepositorInsertArabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229L-pET151
Plasmid#177879PurposeExpresses mouse Talin 1 F2F3-P229L domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Leucine (P229L)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229S-pET151
Plasmid#177878PurposeExpresses mouse Talin 1 F2F3-P229S domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Serine (P229S)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-amylase sensor
Plasmid#186293PurposeExpression alpha-amylase sensor in e coliDepositorInsertcpLumiLuc-amylase sensor
UseTagsHisTagExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-HSA sensor
Plasmid#186294PurposeExpression Human serum albumin sensor in e coliDepositorInsertcpLumiLuc-HSA sensor
UseTagsHisTagExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-TR-E192C-del1-40
Plasmid#180235PurposeExpresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364) with C terminal cysteine for maleimide chemistryDepositorInsertColicin E1
UseTags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T and R domains (r…PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-LanCL1-H219A
Plasmid#154190PurposeTo express LanCL1-H219A in bacterial cellsDepositorInsertLanCL1
UseTagsHexahistidineExpressionBacterialMutationHis219->Ala219PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-LanCL1-C322A
Plasmid#154191PurposeTo express LanCL1-C322A in bacterial cellsDepositorInsertLanCL1
UseTagsHexahistidineExpressionBacterialMutationCys322->Ala322PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15 PemrR-gfp biosensor
Plasmid#185793PurposeThe pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.DepositorInsertpEmrR-gfp
UseTagsEGFPExpressionBacterialMutationPromoterPemrRAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b MOSPD2 [315-445]
Plasmid#186474PurposeExpression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteriaDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseTags6HISExpressionBacterialMutationMSP domain [315-445]PromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b MOSPD2 RD/LD [282-490]
Plasmid#186470PurposeExpression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteriaDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseTags6HISExpressionBacterialMutationMSP domain [282-490]PromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only