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pDSARN-vasa-eSpCas9sv40

Plasmid

#173670

Purpose

eSpCas9 expression vector for Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Insert

eSpCas9 with Anopheles vasa promoter and SV40 terminator

Use

CRISPR

Tags

Expression

Insect

Mutation

mutations in Cas9 reducing off-target activity (S…

Promoter

vasa

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4A RNAiR

Plasmid

#115172

Purpose

expresses RNAi-resistant Sema4A with N-terminal myc tag

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

Sema4A (Sema4a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Silent mutation between amino acids in ORF (401-4…

Promoter

CMV

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode81

Plasmid

#83758

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode81

Use

Retroviral

Tags

Expression

Mutation

deletion of T in starting ATG of GFP (please see …

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode71

Plasmid

#83748

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode71

Use

Retroviral

Tags

Expression

Mutation

deletion of AA5-233 in GFP (please see depositor …

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKAsub-YPet-Kras

Plasmid

#138206

Purpose

Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

PKAsub-YPet-Kras

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pBMN-mCherry-Parkin

Plasmid

#59419

Purpose

to make mCherry-Parkin stable cell line with retrovirus

Depositor

Richard Youle

Article

Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

Insert

mCherry-Parkin (PRKN Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBMN-YFP-Parkin

Plasmid

#59416

Purpose

to make YFP-Parkin stable cell line with retrovirus

Depositor

Richard Youle

Article

Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

Insert

YFP-Parkin (PRKN Human)

Use

Retroviral

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pTW040

Plasmid

#64769

Purpose

For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

Cre-EBD78

Use

Tags

fused to the human Estrogen Binding Domain (EBD),…

Expression

Yeast

Mutation

Promoter

TDH3

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_EGFP

Plasmid

#92194

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pB-CAG-FLEX-F14F15-BFP-(mCherry)'

Plasmid

#201831

Purpose

piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Inserts

CAG
BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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modRNAc0-ABE8e-P2A-GFP

Plasmid

#178177

Purpose

IVT template plasmid for making ABE8e-P2A-GFP modRNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

ABE8e

Use

Ivt template plasmid

Tags

P2A-GFP

Expression

Mutation

Promoter

T7 promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-miniTurbo-L2

Plasmid

#127352

Purpose

gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, V5, and YFP

Expression

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSS146

Plasmid

#64770

Purpose

For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

Cre-EBD78

Use

Tags

fused to the human Estrogen Binding Domain (EBD),…

Expression

Yeast

Mutation

Promoter

TDH4

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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neurod1:cre; cmlc2:EGFP

Plasmid

#173480

Purpose

cre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP maker

Depositor

John Rawls

Article

Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

Insert

Cre

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLC242-GFP-metap2

Plasmid

#100518

Purpose

Expresses GFP tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-Turbo-L2

Plasmid

#127350

Purpose

gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, V5, and YFP

Expression

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag HA PGC-1 alpha

Plasmid

#14426

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
Lenti-dual-CRISPR-U6-H1(pBP43)

Plasmid

#218930

Purpose

The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

Depositor

Baoxu Pang

Article

Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6 and H1

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-Turbo-NES-L2

Plasmid

#127349

Purpose

gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Expression

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-V5-KDM3A

Plasmid

#82331

Purpose

lentiviral expression of KDM3A

Depositor

Luke Gaughan

Article

Wade et al Nucleic Acids Res. 2015 Jan;43(1):196-20

Insert

KDM3A (KDM3A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

two silent mutations to make it siRNA resistant

Promoter

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only