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Plasmid#221470PurposeGateway cloning of mCherry overexpressionDepositorInsertmCherry
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsNot10_25-707_AF
Plasmid#148678PurposeBacterial Expression of HsNot10_25-707DepositorInsertHsNot10_25-707 (CNOT10 Human)
UseTagsExpressionBacterialMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-hcRed-V5_IDG-K
Plasmid#135239PurposeGateway destination clone of hcRed (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInserthcRed-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-eGFP_IDG-K
Plasmid#135265PurposeGateway destination clone of eGFP (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-eGFP
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-hcRed_IDG-K
Plasmid#135274PurposeGateway destination clone of hcRed (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-hcRed
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACY.HRF_T7_term_HR1
Plasmid#111981PurposeSub cloning vector position 1DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
TJP3
Plasmid#25310PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTJP3:G508-L808:cH6 (TJP3 Human)
UseTagsHisExpressionBacterialMutationContains amino acids G508-L808PromoterAvailable sinceApril 4, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
Pf-FKBP-TPR
Plasmid#25582PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPf-FKBP-TPR (FKBP35 Plasmodium Falciparum)
UseTagsHisExpressionBacterialMutationContains amino acid residues 128-304.PromoterAvailable sinceOct. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
SGF29 (3MEW)
Plasmid#25320PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSGF29_23 (SGF29 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 129-291PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
LentiCRISPRv2GFP
Plasmid#82416Purpose3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning siteDepositorHas ServiceCloning Grade DNAInsertGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFS (P2A)Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA
Plasmid#121750PurposepMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsert3x HA epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCXMG
Plasmid#204409PurposeExpression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI siteDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromotertacAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE
Plasmid#138147PurposeExpresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInsertCasRx
UseLentiviralTagsNLSExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE
Plasmid#138148PurposeExpresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInsertCasRx
UseLentiviralTagsNESExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB501
Plasmid#74843PurposeGateway cloning compatible binary vector for simple cloning of gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB401
Plasmid#74795PurposeGateway cloning compatible binary vector for simple cloning of gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-nsp1-c3xFLAG
Plasmid#215694PurposeProduces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp1
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-nsp2-c3xFLAG
Plasmid#215695PurposeProduces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp2
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-nsp3-c3xFLAG
Plasmid#215696PurposeProduces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp3
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-nsp4-c3xFLAG
Plasmid#215697PurposeProduces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp4
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only