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Plasmid#67987PurposeMouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassetteDepositorInsertEF1a-hCas9IRESneopA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G
Plasmid#204189PurposeDoxycycline inducible lentiviral vector for human GATA6 overexpressionDepositorInsertGATA6 (GATA6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pv6_MECP2_MBD
Plasmid#179407PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBDDepositorInsertMECP2_MBD (Mecp2 Mouse)
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMIG-hNFATc2/C - AVITEV
Plasmid#74058Purposeretroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1+_FH-AGO2-5xA
Plasmid#92007PurposeExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
UseTagsFLAG-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)PromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Dnmt3b1-Poly(A)-PuroR
Plasmid#65556PurposePlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsmCherryExpressionMammalianMutationisoform 1PromoterAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)13
Plasmid#127526PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Isl1
Plasmid#72696PurposeDoxycyline regulated expression of Isl1 in mammalian cellsDepositorInsertIslet1 (Isl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Dnmt3b6-Poly(A)-NeoR
Plasmid#65554PurposePlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsmCherryExpressionMammalianMutationisoform 6PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_FH-AGO2-5xE
Plasmid#92008PurposeExpresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]DepositorInsertAGO2 (AGO2 Human)
UseTagsFLAG-HAExpressionMammalianMutationS824E, S828E, T830E, S831E, S834E (5xE)PromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y
Plasmid#169469PurposeEncodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSAM2_mCherry_Irx4
Plasmid#72692PurposeDoxycyline regulated expression of Irx4 in mammalian cellsDepositorInsertIrx 4 (IRX4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP8.1
Plasmid#189846PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.DepositorTypeEmpty backboneUseCre/LoxTagsmRFP and tagBFPExpressionMutationPromoterbeta ActinAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75236PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only