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Plasmid#134997PurposeInterspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertInters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP498
Plasmid#134998PurposeClustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertClust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP650
Plasmid#135000PurposeVP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoRDepositorInsertpUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
UseLentiviral and Synthetic BiologyTagsDpnI(aa146-254), V5 tag, and VP-64ExpressionMammalianMutationDpnI truncated to aa146-254PromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLV-Rtn2b-R60f-mCherry
Plasmid#186607PurposeThird generation lentiviral vector expressing Reticulon 2 disease mutant R60f fused to mCherryDepositorInsertHuman Reticulon 2 mutant R60f (RTN2 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationc.178insC (R60f)PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-NTD136-271-mNeon
Plasmid#186604PurposeEncodes human Reticulon 2 isoform B N-terminal domain aa 136 to 271 fused to mNeonGreenDepositorInsertHuman Reticulon 2 isoform B amino acids 136 to 271 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcry:mCherry,-600unc:cct3GFP
Plasmid#172528PurposealphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.DepositorInsertcct3-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3
Plasmid#128758PurposeExpresses PAPD4 gRNA for CRISPRiDepositorInsertPAPD4 sgRNA #3 (TENT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1
Plasmid#128756PurposeExpresses PAPD4 gRNA for CRISPRiDepositorInsertPAPD4 sgRNA #1 (TENT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCB-hDot1Lwt
Plasmid#74173PurposeFor packaging hDot1Lwt to retrovirus.DepositorInserthDot1L (DOT1L Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS rat IRS-3
Plasmid#11361DepositorInsertIRS-3 (Irs3 Rat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
DL146_plenti_TRE_GFP-iPAK4
Plasmid#187446PurposeTet-On inducible expression of eGFP-iPAK4 in a lentiviral backboneDepositorInsertINKA-box-Pak4
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
p4458 FLAG-hPLIC-1
Plasmid#8663DepositorInserthPLIC-1 (UBQLN1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-histone H3
Plasmid#179699Purposevisualization of nucleus/chromosomeDepositorInsertHistone H3 (Hist1h3h Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-α5last
Plasmid#160270PurposeExpresses a concatemeric construct of a α4:β2:α5 (2:2:1) nAChRDepositorInsertα5last concatemer
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-α5first
Plasmid#160269PurposeExpresses a concatemeric construct of a α4:β2:α5 (2:2:1) nAChRDepositorInsertα5first concatemer
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
DL145_plenti_TRE_iPAK4
Plasmid#187445PurposeTet-On inducible expression of iPAK4 in a lentiviral backboneDepositorInsertINKA-box-Pak4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
DL147_plenti_TRE_HT-iPAK4
Plasmid#187447PurposeTet-On inducible expression of HaloTag-iPAK4 in a lentiviral backboneDepositorInsertINKA-box Pak4
UseLentiviralTagshalo tagExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
p6239 pGL4-HPV16-LCR (nt 7000-85)-luciferase
Plasmid#32889DepositorInsertLCR HPV16
UseTagsExpressionMutationPromoterLCRAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -