Addgene

We narrowed to 3,250 results for: 376

Showing: 3061 - 3080 of 3250 results

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pET30a-SRAP

Plasmid

#84524

Purpose

Expresses murine SRAP domain (250 aa) in bacteria

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

SRAP domain (Hmces Mouse)

Use

Tags

6xHis and S-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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KHBD00702

Plasmid

#39738

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11033 (Kdm2 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-VcRibB

Plasmid

#187977

Purpose

Expresses 3,4-dihydroxy-2-butanone 4-phosphate synthase RibB from Vibrio cholerae

Depositor

Audrey Lamb

Article

Kenjic et al J Am Chem Soc. 2022 Jul 20;144(28):12769

Insert

VcRibB

Use

Tags

Hexahistag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pNL2114 pARF19 GG0-GG1

Plasmid

#195909

Purpose

Level 0 pARF19 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF19 (ARF19 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I16 tUBQ1 GG5-GG6

Plasmid

#195898

Purpose

Level 0 tUBQ1 terminator with GG5 GG6 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

tUBQ1 (UBQ1 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I15 tUBQ1 GG4-GG6

Plasmid

#195897

Purpose

Level 0 tUBQ1 terminator with GG4 GG6 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

tUBQ1 (UBQ1 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_ZDHHC6-1/1

Plasmid

#91505

Purpose

Protein expression and purification of human SH3 domain construct ZDHHC6-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ZDHHC6-1/1 (ZDHHC6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00523

Plasmid

#39623

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11966 (CG11966 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00571

Plasmid

#39657

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11984 (CG11984 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00541

Plasmid

#39636

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8159 (CG8159 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00537

Plasmid

#39633

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9793 (ranshi Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00448

Plasmid

#39561

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11762 (CG11762 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00136

Plasmid

#34290

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9797 (M1BP Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00034

Plasmid

#34196

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8359 (CG8359 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00089

Plasmid

#34245

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8145 (CG8145 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
Plxnd1-Fc-His

Plasmid

#72132

Purpose

Expresses the extracellular region of the PlexinD1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnd1 (Plxnd1 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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betaTrCP mVenus mutant reporter

Plasmid

#192459

Purpose

Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

Depositor

Steven Cappell

Article

Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

Insert

CdC25B

Use

Lentiviral

Tags

SV40 NLS and mVenus

Expression

Mammalian

Mutation

aa198-338 of CDC25B. betaTrCP degron sequence (aa…

Promoter

EF1alpha

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI

Plasmid

#192112

Purpose

Expresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Insert

SARS-CoV-2-S2P-BA.4/5 (S )

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK2

Plasmid

#197117

Purpose

Expression of Myc-tagged PSK2 / TAOK1 in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

Insert

TAOK1 (TAOK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pB-bsd-Flag-CNP

Plasmid

#126608

Purpose

Expresses N-terminal Flag-tagged human CNP

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

2'3' cyclic nucleotide phosphodiesterase (CNP Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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