Addgene

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pBAD33(EFG)

Plasmid

#187087

Purpose

Medium expression of subunits EFG

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoEFG

Use

Tags

HA tag nuoG

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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RTA

Plasmid

#64029

Purpose

Expression of Ricin A Chain (RTA) in E. coli

Depositor

Vern Schramm

Article

Ho et al Proc Natl Acad Sci U S A. 2009 Dec 1;106

Insert

Ricin A Chain

Use

Tags

none

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pGC180

Plasmid

#19616

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(attR1-attR2)::FLP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt, destination vector

Tags

Expression

Worm

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pGC249

Plasmid

#19676

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R4-R3)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGC236

Plasmid

#19675

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Mutation

Promoter

Available since

Feb. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pGC89

Plasmid

#19642

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<1xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC319

Plasmid

#19677

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R4-R3)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Mutation

Promoter

Available since

March 17, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV2-CAGIG-WGA-mCherry

Plasmid

#224119

Purpose

To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.

Depositor

Pantelis Tsoulfas

Insert

WGA-mCherry

Use

AAV

Tags

WGA and mCherry

Expression

Mammalian

Mutation

All mutations related to the WGA in this construc…

Promoter

CAGIG

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Neo-rTOM20-TagBFP

Plasmid

#210332

Purpose

Overexpression of rTOM20

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rTOM20 (Tomm20 Rat)

Use

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD33(A-H*HA-N)

Plasmid

#187086

Purpose

Medium expression of Complex I with subunits N and H -HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Use

Tags

HA tags nuoN, nuoH

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pUG-294

Plasmid

#104723

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC2_EL3

Use

Tags

HIS-Ubq, HA-E2

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGC452

Plasmid

#19627

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phlh-12

Use

RNAi; Flp/frt

Tags

Expression

Worm

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pGC90

Plasmid

#19643

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-APEX2-NIK3x

Plasmid

#129274

Purpose

Expression of APEX2 in the nucleolus

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

GFP-APEX2-NIK3x

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-ERT2CreERT2

Plasmid

#13777

Purpose

Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-CreERT2

Plasmid

#14797

Purpose

Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre-ERT2 fusion protein

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pB-mTSPAN4-mCherry

Plasmid

#210330

Purpose

Overexpression of mTSPAN4

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mTSPAN4 (Tspan4 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Neo-GFP-hMyo19 iso2

Plasmid

#210333

Purpose

Overexpression of hMyo19

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

hMyo19 (MYO19 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pB-mTSPAN4-GFP

Plasmid

#210329

Purpose

Overexpression of mTSPAN4

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mTSPAN4 (Tspan4 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC185

Plasmid

#19624

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge