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lenti-KRAS-G12C-tmpknot-epegRNA

Plasmid

#214094

Purpose

Lentiviral vector expressing epegRNA to induce KRAS G12C mutation

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

KRAS G12C epegRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-KRAS-G13A-tmpknot-epegRNA

Plasmid

#214095

Purpose

Lentiviral vector expressing epegRNA to induce KRAS G13A mutation

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

KRAS G13A epegRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-hP53DD

Plasmid

#214084

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

PEmax-P2A-hP53DD (TP53 Synthetic, SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-Chrimson-TdTomato

Plasmid

#209202

Purpose

Cre-dependent expression of mCherry-tagged Chrimson; Optogenetics

Depositor

Larry Zweifel

Article

Soden et al Nat Neurosci. 2020 Aug;23(8):968-980. do

Insert

Chrimson

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_Left-ND6-1397N

Plasmid

#212952

Purpose

Expresses TALED_Left-ND6-1397N in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Left-ND6-1397N

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_Right-Cox3.1-1397C-AD.

Plasmid

#212953

Purpose

ExpressesTALED_Right-Cox3.1-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Right-Cox3.1-1397C-AD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_Right-ND6-1397C-AD

Plasmid

#212956

Purpose

Expresses TALED_Right-ND6-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Right-ND6-1397C-AD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_Left-Cox3.1-1397N.

Plasmid

#212950

Purpose

Expresses TALED_Left-Cox3.1-1397N. in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Left-Cox3.1-1397N

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mTagBFP2-FRB-Rab7

Plasmid

#214273

Purpose

Mammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chemical Biology

Insert

mTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV_JAK3_IRES_BFP

Plasmid

#206291

Purpose

Expresses the wild type form of human JAK3.

Depositor

Charles de Bock

Article

Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

Insert

JAK3 (JAK3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pWPI-LUC7L-3xFLAG

Plasmid

#201640

Purpose

Over-expression of 3xFLAG-tagged human LUC7L

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L (LUC7L Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RRVQ

Plasmid

#200188

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing

Depositor

Yiping Qi

Article

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-RRVQ

Use

CRISPR; Gateway compatible lbcas12a-rrv entry clo…

Tags

NLS

Expression

Plant

Mutation

G146R, D156R, R182V, E795Q

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_1G11F2-3 light chain

Plasmid

#198254

Purpose

recombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

light chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)

Use

Tags

IL10 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

Plasmid

#198599

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-143-144

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)Rigor-L-2xHA

Plasmid

#193718

Purpose

Expression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)Rigor-L-2xHA (KIF5B Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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p3X-UASTattB

Plasmid

#189859

Purpose

Fly cloning and transformation

Depositor

Jung Hwan Kim

Article

Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DSUP_RAMVA (pBS0353)

Plasmid

#185189

Purpose

For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DSUP_RAMVA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_FLAG-NFL(WT)

Plasmid

#182659

Purpose

Expresses wild-type mouse neurofilament light chain with an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

wild type mouse neurofilament light chain (Nefl Mouse)

Use

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-RIIα

Plasmid

#168492

Purpose

Mammalian expression of mouse PKA regulatory subunit Iiα, untagged.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

mouse PKA regulatory subunit IIα, untagged

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A mmPing20

Plasmid

#145788

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20

Use

Tags

Expression

Yeast

Mutation

T162C, T258A, T287C, T300A, T304A, T310A, G372A

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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