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Plasmid#48811PurposeThis report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.DepositorInsertnAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsExpressionMammalianMutation*PromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-D176H
Plasmid#69919PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationD176HPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas6
Plasmid#106274PurposeEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasE
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA HA FAK Y577F
Plasmid#50507DepositorInsertprotein tyrosine kinase, mutated
UseTagsHAExpressionMammalianMutationY577FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hDDB2-K244E-V5
Plasmid#167471Purposeectopic expression of hDDB2 in mammalian cellsDepositorInsertDDB2 (DDB2 Human)
UseTags6xHis and V5ExpressionMammalianMutationK244EPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp7
Plasmid#157689Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp7 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-GATA2-DNzf
Plasmid#1291DepositorInsertGATA2 (GATA2 Human)
UseTagsExpressionMammalianMutationS263G and M434VPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
NLS-4LNDDE488Q-pCDNA3.1(+)
Plasmid#172314PurposeExpresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)DepositorInsertLambda N peptide - human ADAR2 Deaminase Domain E488Q
UseTags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianMutationchanged the Glutamic acid 488 in the Deaminase Do…PromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RIPK3 K55R
Plasmid#78807PurposeTo overexpress RIPK3 K55R in Mammalian CellsDepositorInsertRIPK3 (RIPK3 Human)
UseTagsHAExpressionMammalianMutationK55RPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 GATA1 K137R
Plasmid#85694PurposeMammalian expression of mouse GATA1 with K137R mutationDepositorInsertGATA1 (Gata1 Mouse)
UseTagsExpressionMammalianMutationK137R (SUMO-1 attachment site)PromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP2
Plasmid#18997DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsHisExpressionMammalianMutationwild typePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp15-HF
Plasmid#157722Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoterDepositorInsertNsp15-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3-HA/Bmal L95E
Plasmid#47344PurposePcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003DepositorInsertBMAL (Bmal1 Mouse)
UseTagsHaExpressionMammalianMutationL95EPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA FLAG mTOR ST
Plasmid#22999DepositorInsertmTOR (MTOR Human)
UseTagsFLAGExpressionMammalianMutationS2035TPromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO DNAJB2b
Plasmid#19470DepositorInsertDNAJB2b (DNAJB2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(K8,9A)-mPAGFP
Plasmid#168493PurposeMammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.2-IgG1
Plasmid#149667PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DKAR-Nuc
Plasmid#45499DepositorInsertDKAR-Nuc
UseTagsCFP, NLS, and YFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HRPT2 136X
Plasmid#11049DepositorInsertHRPT2 136X (CDC73 Human)
UseTagsflagExpressionMammalianMutationtruncation mutant with a stop codon introduced at…PromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Q842gp120-8his
Plasmid#100932PurposeMammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strainDepositorInsertHIV-1 (Q842.d12) Env
UseTagsCD5 leader sequence and Purification tag after K5…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only