-
Plasmid#186378PurposeDestination vector for the expression of a fluorescent fusion of N-ter mCherry and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
-
pmCherry2-CD86-mEYFP(Q69K)-mEGFP
Plasmid#190751PurposeExpression of triple tagged CD86 with mCherry2, mEYFP(Q69K), and mEGFP in mammalian cells.DepositorInsertCD86 (CD86 Human)
UseTagsmCherry2, mEGFP, and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry
Plasmid#181854PurposemCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.DepositorUseTagsmCherryExpressionMammalianMutationHistidine 33 in DnaJB1 changed to glutamine.PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-tetO-mCherry-attB53
Plasmid#183612PurposeRMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInserttetO-mCherry
UseRmceTagsExpressionMutationPromotertetOAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-42-DExCon-antiGFPnanobody-mCherry-R11a
Plasmid#179902PurposeDonor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry moduleDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsantiGFPnanobody-mCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS3-TeTLc-P2A-mCherry
Plasmid#178708PurposeAAV vector for Flp-dependent transgene expression of TeTLc-P2A-mCherry in neurons under the control of the hSyn1 promoter (Kugler et al 2003)DepositorInsertTeTLc-P2A-mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP
Plasmid#182853PurposeHetero-trimer expression vector with plasma membrane localization signalDepositorInsertmyr-palm-mEYFP-mCherry2-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-mCherry-Kir2.1-HA-tvmvs-RXR-V2
Plasmid#179707PurposeTVMVP-inducible mCherry-conjugated Kir2.1 RELEASEDepositorInsertKir2.1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66
Plasmid#168787PurposeDonor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.DepositorTypeEmpty backboneUseCre/Lox and Synthetic Biology; Expression in fila…TagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_EPV-CXCL9-mCherry-SBP
Plasmid#170678PurposeExpresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.DepositorInsertsUseTagsSBP and mCherryExpressionMammalianMutationChanged threonine 23 to glutamate (T23E)PromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/6xHis mCherry F30 Broccoli
Plasmid#169234PurposeConstitutive T7 RNAP-mediated expression of 6xHis-mCherry with Broccoli RNA aptamer.DepositorInsert6xHis mCherry F30-Broccoli
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-TREX1 g3 Cas9-T2A-mCherry
Plasmid#164252PurposeTranscription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsgTREX1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dH85E-cs-TEV-STREP
Plasmid#171838PurposeMammalian expression of human WIPI2d H85E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationH85EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dK88E-cs-TEV-STREP
Plasmid#171839PurposeMammalian expression of human WIPI2d K88E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationK88EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dI92E-cs-TEV-STREP
Plasmid#171840PurposeMammalian expression of human WIPI2d I92E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationI92EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dC93E-cs-TEV-STREP
Plasmid#171841PurposeMammalian expression of human WIPI2d C93E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationC93EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dR108E-cs-TEV-STREP
Plasmid#171842PurposeMammalian expression of human WIPI2d with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationR108EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dR125E-cs-TEV-STREP
Plasmid#171843PurposeMammalian expression of human WIPI2d R108E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationR125EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP
Plasmid#171844PurposeMammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationK128EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-BiP-Nb127D01-mCherry-KDEL
Plasmid#171575PurposeExpresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membraneDepositorInsertBiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
UseTagsBiP signal peptide and mCherry-KDELExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only