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Plasmid#198542PurposeExpresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-FCPF
Plasmid#220131PurposeExpresses dCas9-FCPF in mammalian cellsDepositorInsertdCas9-FCPF (cas9 S. pyogenes, Synthetic)
UseTagsFlag and HAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-dCas9-FCPF
Plasmid#220133PurposeExpresses dCas9-FCPF in E.coliDepositorInsertdCas9-FCPF (cas9 S. pyogenes, Synthetic)
UseTags6xHisExpressionBacterialMutationFCPF sequence inserted on C-terminalPromoterT7Available sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-RIPLET ΔPRY/SPRY
Plasmid#218516PurposeRIPLET lacking C-terminal PRY/SPRY domain in pcDNA3.1DepositorInsertRIPLET delta PRY/SPRY domain (RNF135 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M1_YLVV
Plasmid#197081PurposeThis plasmid contains the coding sequence for signaling peptide M1, which contains the YLVV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M1, which contains the YLVV signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M2_YVPM
Plasmid#197082PurposeThis plasmid contains the coding sequence for signaling peptide M2, which contains the YVPM signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M2, which contains the YVPM signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M3_YENL
Plasmid#197083PurposeThis plasmid contains the coding sequence for signaling peptide M3, which contains the YENL signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M3, which contains the YENL signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M4_YVTM_YQNQ
Plasmid#197084PurposeThis plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M5_YESP
Plasmid#197085PurposeThis plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M6_ITYAAV
Plasmid#197086PurposeThis plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M7_PVQP
Plasmid#197087PurposeThis plasmid contains the coding sequence for signaling peptide M7, which contains the PVQP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M7, which contains the PVQP signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M8_RSTKP
Plasmid#197088PurposeThis plasmid contains the coding sequence for signaling peptide M8, which contains the RSTKP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M8, which contains the RSTKP signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M9_PVQE
Plasmid#197091PurposeThis plasmid contains the coding sequence for signaling peptide M9, which contains the PVQE signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M9, which contains the PVQE signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M10_PQQAT
Plasmid#197093PurposeThis plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M11_PQEINF
Plasmid#197094PurposeThis plasmid contains the coding sequence for signaling peptide M11, which contains the PQEINF signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M11, which contains the PQEINF signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M12_RPTAVEG
Plasmid#197095PurposeThis plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M13_6xSAG
Plasmid#197096PurposeThis plasmid contains the coding sequence for the control peptide M13, which contains a linker with six repeats of serine-alanine-glycine. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CDepositorInsertControl peptide M13, which contains a linker with six repeats of serine-alanine-glycine (SAG)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-npas4l
Plasmid#164654Purposefor the synthesis of zebrafish npas4l mRNADepositorInsertnpas4l (npas4l Zebrafish)
UseMrna synthesisTagsExpressionMutationPlease see depositor commentsPromoterSP6Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2z-etsrp
Plasmid#164655Purposefor the synthesis of zebrafish etsrp mRNADepositorInsertetsrp (etsrp Zebrafish)
UseMrna synthesisTagsExpressionMutationPromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only