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Showing: 6081 - 6120 of 9874 results

  12. pEND-321_Cacnes_ori

    Plasmid
    #225603
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225603-plasmid-map-sequence-id-451386

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  13. pEND-400_Cacnes_CAMP2

    Plasmid
    #225606
    Purpose
    Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225606-plasmid-map-sequence-id-451402

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  14. pEND-418_Cacnes_LysA

    Plasmid
    #225607
    Purpose
    Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225607-plasmid-map-sequence-id-451378

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  15. pEND-443_Cacnes_HisB

    Plasmid
    #225608
    Purpose
    Suicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225608-plasmid-map-sequence-id-451366

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  16. pEND-446_Cacnes_ProA

    Plasmid
    #225609
    Purpose
    Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225609-plasmid-map-sequence-id-451370

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  17. pEND-320_Cacnes_ori

    Plasmid
    #225602
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225602-plasmid-map-sequence-id-451377

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  20. FKBP-LBR(1-245)-GFP

    Plasmid
    #222425
    Purpose
    To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR(1-245aa) (LBR Human)
    Use
    Tags
    FKBP and GFP
    Expression
    Mammalian
    Mutation
    Truncation (Includes aa 1-245)
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    222425-plasmid-map-sequence-id-439254

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  21. LBR(R583Q)-FKBP-GFP

    Plasmid
    #222423
    Purpose
    To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR(R583Q) (LBR Human)
    Use
    Tags
    FKBP-GFP
    Expression
    Mammalian
    Mutation
    R583Q
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222423-plasmid-map-sequence-id-439219

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  22. LBR(N547D)-FKBP-GFP

    Plasmid
    #222422
    Purpose
    To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR(N547D) (LBR Human)
    Use
    Tags
    FKBP-GFP
    Expression
    Mammalian
    Mutation
    N547D
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222422-plasmid-map-sequence-id-439218

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  25. pSCL758

    Plasmid
    #219504
    Purpose
    Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.
    Insert
    msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219504-plasmid-map-sequence-id-435244

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  27. PB-3xCTCF-TetO

    Plasmid
    #213023
    Purpose
    piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase
    Depositor
    Luca Giorgetti
    Article
    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do
    Insert
    3xCTCF-TetO
    Use
    Mouse Targeting; Piggybase integration (transposo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    213023-plasmid-map-sequence-id-428697

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  39. mEos3.2trLATo

    Plasmid
    #203746
    Purpose
    Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLATo (Lat Mouse)
    Use
    Tags
    ER translocation signal - mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203746-plasmid-map-sequence-id-403345

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Showing: 6081 - 6120 of 9874 results