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Plasmid#102556Purposeexpresses cytoplasmic ATP sensor in neuronsDepositorInsertiATPSnFR1.0
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e-NG
Plasmid#181754PurposeMMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e-NG
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRU295
Plasmid#167694PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-hEF1a-MG
Plasmid#175504PurposeAll-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elementsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1aAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI
Plasmid#201683PurposeAll-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.DepositorInsertNme2-ABE8e-i1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU1aAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)
Plasmid#199605PurposeExpression of cell permeable opAsCas12a in E. Coli.DepositorInsertopCas12a-T8N
UseCRISPRTags6xHis, Twin-strep, SUMOExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Alive
Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
UseTagsHisExpressionBacterialMutationWt genePromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-GRASP55
Plasmid#137708PurposeEukaryotic expression of rat GRASP55 full-lengthDepositorInsertGRASP55
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_NSP4ss_SerM_NucA-His
Plasmid#218190PurposeBacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)DepositorInsertnucA
UseTagsHis and NSP4 signal secretion peptideExpressionBacterialMutationcodon optimized ecoli residues - 22-266PromoterT7Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL2-GAL4-UAS-Luc
Plasmid#33020DepositorInsert6X GAL4-UAS
UseTagsLuciferaseExpressionMutationPromoterAvailable sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP10
Plasmid#22543DepositorInsertUSP10 (USP10 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceJan. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTM1_VP30_ZEBOV
Plasmid#69119PurposeEncodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoterDepositorInsertVP30 ZEBOV (VP30 )
UseT7TagsExpressionMutationPromoterT7Available sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-OGT_opt
Plasmid#190858PurposeFor mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTagsExpressionMammalianMutationCodon optimized for expression in human cells.PromoterCMVAvailable sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTriEx-mCherry-Zdk1
Plasmid#81057Purposemammalian expression of Zdk1DepositorInsertZdk1
UseTags6x His tag, FLAG, and mCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI
Plasmid#201684PurposeAll-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.DepositorInsertNme2-ABE8e-i1 (V106W)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU1aAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-8
Plasmid#208786PurposeInducibly expresses human Caspase 8 fused to light-activatable Cry2DepositorInsertCaspase 8 (CASP8 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationCARD domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDusk
Plasmid#43795PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-LOV2
Plasmid#81009PurposeExpression of LOV2 anchored to mitochondriaDepositorInsertLOV2
UseTagsNTOM20ExpressionBacterial, Insect, and Mamm…MutationPromoterCMV, p10Available sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Wnk1
Plasmid#38779DepositorInsertWnk1 (Wnk1 Rat)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 20, 2013AvailabilityAcademic Institutions and Nonprofits only