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Plasmid#147589PurposeInsect Expression of DmeIF4Ga_578-650-DmCup_417-1133DepositorInsertDmeIF4Ga_578-650-DmCup_417-1133 (eIF4G1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4Ga-dsRNAres_I
Plasmid#146577PurposeInsect Expression of DmeIF4GaDepositorInsertDmeIF4Ga (eIF4G1 Fly)
UseTagsExpressionInsectMutationone non silent mutation K340R compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_578-634-GB1_AG
Plasmid#148744PurposeBacterial Expression of DmeIF4G_578-634DepositorInsertDmeIF4G_578-634 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTV-Flnc-5prime-1000-24MS96T-3F_NeoR
Plasmid#174881PurposeTargeting vector for generating Flnc STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Flnc
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR
Plasmid#174883PurposeTargeting vector for generating Pou5f1 STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Pou5f1
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTV-Usp5-5prime-1000-24MS96T-3F_NeoR
Plasmid#174885PurposeTargeting vector for generating Usp5 STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Usp5
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR
Plasmid#174886PurposeTargeting vector for generating Wnk1 STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Wnk1
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
TERTpWT_Gluc_CCR5
Plasmid#192270PurposeDonor template for insertion of hTERT promoter driven Gaussia luciferase reporterDepositorInsertPromoter Reporter Insert within donor template
UseCRISPRTagsExpressionMutationPromoterTERT promoter driving expression of gaussiaAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TERTp124_Gluc_CCR5
Plasmid#192271PurposeDonor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporterDepositorInsertPromoter Reporter Insert within donor template
UseCRISPRTagsExpressionMutation-124 C to TPromoterTERT promoter driving expression of gaussiaAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TERTp146_Gluc_CCR5
Plasmid#192272PurposeDonor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporterDepositorInsertPromoter Reporter Insert within donor template
UseCRISPRTagsExpressionMutation-146 C to TPromoterTERT promoter driving expression of gaussiaAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pRB116.2
Plasmid#181946PurposeaTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129DepositorInsertUseTagsmNeonGreenExpressionBacterialMutationPromoterTorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB120.2
Plasmid#181947PurposeaTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129DepositorInsertUseTagsmNeonGreenExpressionBacterialMutationPromotermNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB133.2
Plasmid#181950PurposeaTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirKDepositorInsertPhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 PhoP-Salmonella enterica subsp. enterica serovar Typhimurium, Synthetic)
UseTagsmNeonGreenExpressionBacterialMutationPromoterPhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z
Plasmid#148160PurposeInsect Expression of Dm4E-T_1-511-LLFWKtoAAAAADepositorInsertDm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)
UseTagsExpressionInsectMutationalias: 4E-T; one non silent mutation L127S, annot…PromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-CBP(1-287)
Plasmid#182839PurposeCBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)DepositorInsertCBP1-287
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-GB1_Z
Plasmid#148180PurposeBacterial Expression of DmThor_50-83DepositorInsertDmThor_50-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only