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Plasmid#12593DepositorInsertN-term truncated mut of Raf-1 (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutation22W, N-terminally truncatedPromoterAvailable sinceSept. 21, 2006AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP(iL)-Grx1(human)
Plasmid#65002PurposeYeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1DepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP(iL)ExpressionYeastMutationPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-CUL1-3'UTR
Plasmid#97156PurposeFor dual luciferase report assayDepositorInsertCUL1 3'UTR (CUL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3
Plasmid#49208Purposeexpresses Flag tagged mouse Tim3DepositorInsertTim3 (Havcr2 Mouse)
UseTagsFLAG and signal sequenceExpressionMammalianMutationno Tim3 signal sequencePromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3CY56
Plasmid#103075PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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MSCVpuro-6xHis_SUMO2
Plasmid#164938PurposeExpresses 6xHis tagged human Small Ubiquitin Like Modifier 2 (SUMO2) wild type cDNADepositorInsertSmall Ubiquitin Like Modifier 2 (SUMO2 Human)
UseRetroviralTags6XHISExpressionMutationPromoterPGK promoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPC1(D786N)-FLAG
Plasmid#164975PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationD786N, insertion L aa15 (please see depositor co…PromoterEF1aAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
B1 Integrin-GFP
Plasmid#186138PurposeFor expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteriaDepositorInsertITGB1 (ITGB1 Human)
UseTagsMBP and mEGFPExpressionBacterialMutationPromoterLacAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b GFP-Rab10 Q68LHis
Plasmid#186015PurposeGFP-Rab10 Q68LHisDepositorInsertGFP-Rab10 Q68LHis (RAB10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pH2R-P2A-mCherry-N1
Plasmid#84331PurposeCo-expresses (untagged) Histamine-2 receptor and mCherryDepositorInsertH2R-P2A-mCherry (HRH2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYL236
Plasmid#173184PurposeCRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 (EZH2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYL214
Plasmid#171031PurposeCRISPR plasmids encodes Cas9 and a sgRNA targeting EZH2 exon 2 - intron 2 junction (sequence: GCAGACGAGCTGATGAAGTAA)DepositorInsertEZH2 (EZH2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRetroX SgK269 WT
Plasmid#68028Purposeexpression of Sgk269 in mammalian cellsDepositorInsertPEAK1 (PEAK1 Human)
UseRetroviralTagsFLAG/myc, HA, and IRES-DsRedExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-FGF21
Plasmid#182407PurposeBacterial expression of Vesicle Nucleating peptide-fgf21 fusionDepositorInsertVNp-fgf21 (FGF21 Human)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ppk301-T2A-QF2 HDR plasmid
Plasmid#130667PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 geneDepositorInsertppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
B1 Integrin
Plasmid#186137PurposeFor expression of recombinant beta1 integrin cytosolic domain in bacteriaDepositorInsertITGB1 (ITGB1 Human)
UseTagsMBP and untranslated His6 after stop codonExpressionBacterialMutationPromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S163/164A
Plasmid#33089DepositorInsertYAP (YAP1 )
UseTagsFlagExpressionMammalianMutationS163A, S164APromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-MYEF2
Plasmid#38069DepositorInsertMYEF2 myelin expression factor 2 (MYEF2 Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only