Addgene

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sTR060

Plasmid

#177370

Purpose

Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

Depositor

Jerome Bonnet, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Insert

Trigger RNA

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p17xQUAS-Luciferase, α-cry:mCherry

Plasmid

#180482

Purpose

Inducible gene expression vector p17xQUAS-Luciferase, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Inserts

Luciferase
17x QUAS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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200 bp Axin2 mutant

Plasmid

#25704

Depositor

Eric Fearon

Article

Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

Insert

Axin 2 promoter (AXIN2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

TCF DNA binding site at bp -108 to -102 mutated …

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pBabe puro H-Ras 12V (37G)

Plasmid

#12589

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

HRas 12V 37G (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

12V, 37G

Promoter

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
His10-B1 Integrin

Plasmid

#186139

Purpose

For expression of recombinant his10-beta1 integrin cytosolic domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

ITGB1 (ITGB1 Human)

Use

Tags

MBP + uncleavable His10

Expression

Bacterial

Mutation

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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mKikGR-Tubulin-6

Plasmid

#57330

Purpose

Localization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Tubulin (TUBA1B Human)

Use

Tags

mKikGR

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19-K1LM04

Plasmid

#103119

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)

Use

CRISPR; Cloning vector

Tags

Expression

Mutation

human codon-optimized

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1162

Plasmid

#111098

Purpose

Expresses FANCA_-_89883048.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA gRNA (FANCA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-coHPDL WT 3xFlag-V5

Plasmid

#174129

Purpose

Expresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Codon optimized

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pmiR-96 cluster promoter

Plasmid

#62517

Purpose

microRNA 183-96-182 cluster promoter luciferase plasmid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2014 Mar;42(5):2988-9

Insert

promoter of microRNA-183-96-182 cluster

Use

Tags

RenSP (optimized renilla luciferase gene)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pUC19-A0LWB3

Plasmid

#103070

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

A0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))

Use

CRISPR; Cloning vector

Tags

Expression

Mutation

human codon-optimized

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1272

Plasmid

#111107

Purpose

Expresses FANCI_+_89787428.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCI gRNA (FANCI Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBabepuro-Willin

Plasmid

#40884

Depositor

Paul Reynolds

Article

Angus et al Oncogene. 2012 Jan 12;31(2):238-50. doi:

Insert

Willin (FRMD6 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
XE252 pCDNA3.1(zeo)-hDsh3-333-716

Plasmid

#16757

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-333-716-Dsh3 (DVL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 333-716

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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XE250 pCDNA3.1 + (zeo)- VSV-KLHL12-Q405X

Plasmid

#16759

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

VSV-KLHL12-Q405X (KLHL12 Human)

Use

Tags

vsv

Expression

Mammalian

Mutation

Q405STOP

Promoter

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ

Plasmid

#16754

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-hDsh3-trunc before PDZ (DVL3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

truncated before PDZ

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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pEBG-MKK4-K129R (GST)

Plasmid

#21564

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Lysine 129 to arginine

Promoter

Available since

July 17, 2009

Availability

Academic Institutions and Nonprofits only
pBabe puro H-Ras 12V (40C)

Plasmid

#12590

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

HRas 12V 40C (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

12V, 40C

Promoter

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
sgp27#1

Plasmid

#102759

Purpose

Expresses sgRNA targeting mouse p27

Depositor

Jin Chen

Article

Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

Insert

p27 (Cdkn1b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST-HA, Pbl-A

Plasmid

#69792

Purpose

Pebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

Depositor

Arno Muller

Article

van Impel et al Development. 2009 Mar;136(5):813-22. doi

Insert

Pebble - Drosophila guanine nucleotide exchange factor

Use

P element-based puast vector for gal4-regulated e…

Tags

HA tag

Expression

Insect

Mutation

Encodes Pbl (NP_729306.1) lacking last 9 amino ac…

Promoter

hsp70 promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only