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hu6-sgCebpb_v1 (opti)

Plasmid

#177247

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpb gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpb_1st

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpb_v2 (opti)

Plasmid

#177248

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpb gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpb_2nd

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpd_v1 (opti)

Plasmid

#177249

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpd gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpd_1st

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

Plasmid

#184636

Purpose

Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gat1 (Slc6a1 Mouse)

Use

AAV

Tags

Expression

Mutation

Gat1 gene with Arginine 69 changed to Lysine, to …

Promoter

EF1a

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV CAG-DO-TC66T-mCherry

Plasmid

#175178

Purpose

Cre-off TVA-mCherry fusion protein with 66T mutation

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

TVA

Use

AAV

Tags

mCherry

Expression

Mutation

E66T

Promoter

CAG

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP]

Plasmid

#153542

Purpose

AAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeQ-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1α1.1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-FLEX-rc [ChromeT-GFP]

Plasmid

#153541

Purpose

AAV-mediated expression of ChromeT-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1α1.1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.2

Plasmid

#149431

Purpose

miniTdg gene, SUMOylation mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

K341A SUMOylation mutant, SNPs of Tdg gene in OLA…

Promoter

Tdg promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.1

Plasmid

#149430

Purpose

miniTdg gene, catalytic mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

N151A catalytic mutant, SNPs of Tdg gene in OLA/1…

Promoter

Tdg promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-pro-alphaf(I)-E2-Crimson

Plasmid

#117660

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf(I)-E2-Crimson

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

Plasmid

#117661

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-alphaf(I)-E2-Crimson

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-Ost1-alphaf(I)-Btl2

Plasmid

#117667

Purpose

Study secretion efficiency of Btl2

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-alphaf(I)-Btl2

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the lipase Btl2 together wit…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus sFRP2

Plasmid

#24696

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2000 Sep . 96(2):183-95.

Insert

secreted frizzled-related sequence protein 2 (sfrp2 Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pJK272

Plasmid

#71562

Purpose

Produces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid acetylenase

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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SEPT5 WT

Plasmid

#27272

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Use

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
GST-SEPT5 WT

Plasmid

#27269

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pJK273

Plasmid

#71563

Purpose

Produces Crepis alpina delta12 fatty acid desaturase (FAD2-2) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid desaturase (form 2)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJK277

Plasmid

#71565

Purpose

Produces Crepis alpina delta15 fatty acid desaturase (FAD3) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta15 fatty acid desaturase

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJK274

Plasmid

#71564

Purpose

Produces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid desaturase (form 3)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1 promoter

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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SEPT5 Mut(S161A/S327A)

Plasmid

#27275

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Use

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Mutation

S161A and S327A

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only