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Plasmid#138260PurposeExpression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter systemDepositorInsertNontargeting sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-FeGFP
Plasmid#138261PurposeDonor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmidDepositorInserteGFP
UseCRISPRTagsExpressionMammalianMutationPromoterNoneAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-ysg(NT)
Plasmid#138263PurposeSubcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.DepositorInsertNontargeting sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
UL79(E48A_L49A)-3xFLAG pCDNA4
Plasmid#129759PurposeExpresses CMV UL79 (E48A_L49A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertUL79 (UL79 Human Cytomegalovirus (HHV-5))
UseTags3xFLAGExpressionMammalianMutationchanged Glutamic acid 48 to Alanine and Leucine 4…PromoterCMVAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_39J
Plasmid#91155PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:barDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3_zfh1_CP-candidate_luc+
Plasmid#86391PurposeLuciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activityDepositorInsertD. melanogaster zfh1 enhancer
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB(KTF)-HA no-pA
Plasmid#82596Purpose3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationW2101FPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
S. aureus DHPS
Plasmid#64079PurposeExpression of Staphylococcus aureus dihydropteroate synthaseDepositorInsertdihydropteroate synthase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5woH
Plasmid#65019PurposeExpresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureusDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_WTnoGirdles
Plasmid#62653PurposeWild-type OmpA with aromatic girdle residues replaced by leucineDepositorInsertOmpA_noGirdles
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationAromatic girdle residues mutated to Leu (W7, W15,…PromoterT7Available sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM-and4 in situ probe
Plasmid#52105Purposeactinodin 4 and4 antisense in situ probe linearize w/ AatII SP6 polDepositorInsertactinodin 4 in situ probe (and4 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM-and2 in situ probe
Plasmid#52103Purposeactinodin 2 and2 antisense in situ probe linearize w/ ApaI SP6 polDepositorInsertactinodin 2 in situ probe (and2 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM-and3 in situ probe
Plasmid#52104Purposeactinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 polDepositorInsertactinodin 3 in situ probe (and3 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-5'UTR-MAVS-ORF1(KaganE49)
Plasmid#52006PurposeExpresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codon 37 nt upstream of the FL MAVS start codonDepositorInsert5utr-MAVS-ORF1mut
UseTagsExpressionMammalianMutationmutation removing the start codon for ORF1PromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
roGFP_iR_pQE30
Plasmid#48634PurposeBacterial expression vector encoding for the 6XHis tagged redox probe roGFPiR (roGFP C147, R147b, C204)DepositorInsertroGFP1
UseTagsHisTagExpressionBacterialMutationinsertion R147BPromoterAvailable sinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryaa
Plasmid#46166PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin (cryaa Zebrafish)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cyprinodon variegatus cryaa
Plasmid#47664PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin (cryaa Zebrafish)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pFA12
Plasmid#46792PurposeTo express a version of Fun30 with a mutated CUE domain in budding yeastDepositorInsertFUN30, CUE domain mutated (FUN30 Budding Yeast)
UseTagsV5ExpressionYeastMutationFP82AAPromoterAvailable sinceAug. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 Y96F
Plasmid#47094DepositorInsertAL-103 Y96F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY96FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only