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Plasmid#180502PurposeMammalian expression of Nluc(W12stop)DepositorInsertNluc(W12stop)
UseTagsFLAGExpressionMammalianMutationW12(UGG) to amber stop codon(UAG)PromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v4-sfGFP
Plasmid#145175PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 4DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.2-sfGFP
Plasmid#149665PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 2.2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
BACE2_pcDNA6.2/EmGFP-Bsd
Plasmid#176942PurposeMammalian expression vector encoding BACE2 and EmGFP-BsdDepositorInsertBACE2 (BACE2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTUT2
Plasmid#60039PurposeMammalian expression of FLAG-tagged mouse TUT2DepositorInsertTUT2 (Papd4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Xl-VSP1-GFP
Plasmid#51883Purposeexpression of Xl-VSP1 -GFP fusion in Xenopus oocytes or mammalian cellsDepositorInsertVSP1 (tpte2 Frog)
UseXenopus oocyte expressionTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ULK2-K39I
Plasmid#97076PurposeExpresses mutant ULK2 in mammalian cellsDepositorInsertULK2 (ULK2 Human)
UseTagsFLAGExpressionMammalianMutationchanged Lysine 39 to IsoleucinePromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EPHB4 Sense
Plasmid#60638PurposeExpresses the human EPHB4 exon 11/12 circular RNADepositorInsertEphrin type B receptor 4 (EPHB4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)MBII-85-5'/3'ss mut
Plasmid#67645PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.DepositorInsertMBII85 snoRNA
UseTagsExpressionMammalianMutation5' and 3' splice sites mutationsPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY112 (pcDNA3.1-huSaCpf1)
Plasmid#92269PurposeExpression of humanized SaCpf1DepositorInserthuSaCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY114 (pcDNA3.1-huPgCpf1)
Plasmid#92273PurposeExpression of humanized PgCpf1DepositorInserthuPgCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY122 (pcDNA3.1-huCPbCpf1)
Plasmid#92298PurposeExpression of humanized CPbCpf1DepositorInserthuCPbCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY118 (pcDNA3.1-huLb4Cpf1)
Plasmid#92294PurposeExpression of humanized Lb4Cpf1DepositorInserthuLb4Cpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:CRY2:nls:VPR
Plasmid#135989PurposeExpresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cellsDepositorInsertsCRY2 domain fused to the VPR activation domain
CRY2 domain fused to the VPR activation domain
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CONK1-lumitoxin
Plasmid#51689PurposeExpression vector for CONK1-lumitoxinDepositorInsertCONK1-lumitoxin
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-VprBP (RARA)
Plasmid#133587PurposeExpresses human VprBP RARA mutant in mammalian cellsDepositorInsertVpr (HIV-1) binding protein (DCAF1 Human)
UseTagsHAExpressionMammalianMutationRARA mutation of human VprBP defective in DDB1 bi…PromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-Sesn1b
Plasmid#61867PurposeTransient overexpression of Flag-Sesn1b (mouse)DepositorInsertSesn1 (Sesn1 Mouse)
UseTagsFlagExpressionMammalianMutationnonePromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-EKAR
Plasmid#181858PurposeGenetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-EKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SLAP-FLAG
Plasmid#74500PurposeOverexpression of SLAP in mammalian cellsDepositorInsertSLAP (SLA Human)
UseTagsExpressionMammalianMutationN18K, G74A, and T149APromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp13_GC3opt
Plasmid#157714Purposemammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertNsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits