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pET11a-TAX-L10-STEPtag

Plasmid

#159375

Purpose

Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

TAX-L10-STEPtag

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-STEPtag-L10-TAX

Plasmid

#159374

Purpose

Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag-L10-TAX

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET11d-FHis-DND1-Y102A

Plasmid

#70082

Purpose

Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (DND1 Human)

Use

Tags

6xHIS and FLAG

Expression

Bacterial

Mutation

Y102A. Codon optimized for bacterial expression

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

Plasmid

#128278

Purpose

Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

Depositor

Anna Sokac

Article

Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

Insert

twinstar (tsr) (tsr Fly)

Use

Tags

6X Histidine tag

Expression

Bacterial

Mutation

Met1 changed to Pro1; Cys77 silent basepair mutat…

Promoter

T7

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-ACP (from S. aureus)

Plasmid

#63686

Purpose

Inducible expression of Acyl Carrier Protein (ACP) from S. aureus

Depositor

Michael Johnson

Insert

ACP

Use

Tags

His

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only
pDRF1-GW ymYPET-T2A-mTurquoise2

Plasmid

#118444

Purpose

Produces equimolar expression of ymYPET and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET-T2A-mTurquoise2

Use

Tags

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL6-V5-His6

Plasmid

#67422

Purpose

Expression of hArl6 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
HOXC13(human) GSTfusion-pET

Plasmid

#8680

Depositor

Corey Largman

Article

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
M26I DJ-1 TEV site pET15b

Plasmid

#60685

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26I DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26I and TEV site was inserted (see comments belo…

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pETNKI-his-3C-LIC-Amp

Plasmid

#108704

Purpose

Expression of a His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

His-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pETNKI-strepII-3C-LIC-Amp

Plasmid

#108707

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-PhCHS-CmCHI

Plasmid

#73397

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
CmCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET28b-NmCas9-Flag H588A

Plasmid

#109032

Purpose

H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Use

Tags

Flag Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-GFP-AURKA_K162M-mCherry

Plasmid

#99881

Purpose

Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

GFP and mCherry

Expression

Bacterial

Mutation

K162M (kinase-dead)

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 R112C/T180C

Plasmid

#66182

Purpose

Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Use

Tags

His6 tag

Expression

Bacterial

Mutation

R112C, T180C

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 E105C/L171C

Plasmid

#66178

Purpose

Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Use

Tags

His6 tag

Expression

Bacterial

Mutation

E105C, L171C

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-192)

Plasmid

#60245

Purpose

Express the RBD of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains aa7-192 of Msi1

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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M26L DJ-1 TEV site pET15b

Plasmid

#60686

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26L DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26L and TEV site was inserted (see comments belo…

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4C-V5-His6

Plasmid

#67423

Purpose

Expression of hArl4c with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4-V5-His6

Plasmid

#67419

Purpose

Expression of hArl4 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

bp 105 A to T; silent mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only