Addgene

We narrowed to 4,749 results for: codon optimized

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pYPQ292-E848A

Plasmid

#129681

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Tags

Expression

Plant

Mutation

E848A; AaCas12b is rice codon optimized

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA6_DT1

Plasmid

#136668

Purpose

Expresses human codon-optimized AcrIIA6 DT1 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA6_DT1

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA6_D1811

Plasmid

#136667

Purpose

Expresses human codon-optimized AcrIIA6 D1811 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA6_D1811

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA6_D1024

Plasmid

#136666

Purpose

Expresses human codon-optimized AcrIIA6 D1024 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA6_D1024

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA5_D4276

Plasmid

#136664

Purpose

Expresses human codon-optimized AcrIIA5 D4276 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA5_D4276

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA5_D1126

Plasmid

#136663

Purpose

Expresses human codon-optimized AcrIIA5 D1126 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA5_D1126

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2-R232Q

Plasmid

#124064

Purpose

Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXlATL2-R232Q

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-001428-QES.c10.L544Y

Plasmid

#123254

Purpose

Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (001428) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations V208M, …

Promoter

CMV

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-25711-QES.c11

Plasmid

#123219

Purpose

Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (25711) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations I181L, …

Promoter

CMV

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-ZM197M-QES.c10

Plasmid

#123224

Purpose

Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (ZM197M) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations V208M, …

Promoter

CMV

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaL-QES.i01.c08

Plasmid

#123214

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations T49D, P…

Promoter

CMV

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-CH111-QES.c13.L544Y

Plasmid

#123267

Purpose

Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (AD8) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations V208M, …

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-DU422-QES.c12

Plasmid

#123250

Purpose

Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations V181L, …

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-B41gp160-V200E

Plasmid

#123246

Purpose

Mammalian expression plasmid for Env from the B41 HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (B41) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutation V200E

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-CH115gp160-QES.c14

Plasmid

#123279

Purpose

Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (CH115) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations V208M, …

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-Q769gp160-QES.03

Plasmid

#100930

Purpose

Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations A200E;F…

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-BaLgp160-QES.01

Plasmid

#100921

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations T49D;P1…

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-BaLgp160-QES.02

Plasmid

#100923

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations T49D;P1…

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-25711gp160-QES.c02

Plasmid

#111842

Purpose

Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (25711.2) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations I181L;V…

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-BaLgp160-QES.i01.c01

Plasmid

#111839

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations T49D;P1…

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge