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pAc5.1B-EGFP-DmTral-R42E_B

Plasmid

#145988

Purpose

Insect Expression of DmTral-R42E

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-R42E (tral Fly)

Use

Tags

Expression

Insect

Mutation

two silent mutations A198C, A387G and a mutation …

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 ATG3 WT

Plasmid

#129292

Purpose

Gateway entry clone encoding wild-type human ATG3 with a stop codon

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 ATG3 K243R

Plasmid

#129294

Purpose

Gateway entry clone encoding human ATG3 K243R

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

changed Lysine 243 to Arginine

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-3A

Plasmid

#114054

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3A (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670A…

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-3D

Plasmid

#114055

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3D (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670D…

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-MIS12-KARD-3D

Plasmid

#114058

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3D (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670D, S676D and T680D

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLAP-MIS12-KARD-3A

Plasmid

#114059

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3A (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670A, S676A and T680A

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hMIB2_#9

Plasmid

#75255

Purpose

retroviral shRNA vector against human MIB2, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against MIB2 (human) (MIB2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)

Plasmid

#61935

Purpose

Expression of hRPN13 (285-407) (Q337A/Q338A) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hRPN13 (285-407) (ADRM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Construct starts at residue 285, residues Q337 an…

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
KHDRBS3_KH_pGEX

Plasmid

#135133

Purpose

Recombinant expression of KHDRBS3 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

KHDRBS3 (KHDRBS3 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise2-IL-1beta-mNeonGreen

Plasmid

#166783

Purpose

FRET analysis (pro-IL-1beta cleavage) in living cells

Depositor

Hao Wu

Article

Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

Insert

mTurquoise2-IL-1b-mNeongreen (IL1B Human)

Use

Lentiviral

Tags

mNeonGreen and mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise2-Caspase-1-mNeonGreen

Plasmid

#166784

Purpose

FRET analysis (pro caspase-1 cleavage) in living cells

Depositor

Hao Wu

Article

Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

Insert

mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)

Use

Lentiviral

Tags

mNeonGreen and mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-PRMT3

Plasmid

#164695

Purpose

Expression of PRMT3 with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Kaniskan et al Angew Chem Int Ed Engl. 2015 Apr 20;54(1

Insert

PRMT3 (PRMT3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp1

Plasmid

#72687

Purpose

Doxycyline regulated expression of Mesp1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 1 (MESP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-GFP

Plasmid

#222996

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-EGFP

Use

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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ZNF9

Plasmid

#156152

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ZNF9 (CNBP Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 N C-V5-tag

Plasmid

#179987

Purpose

Mammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 N

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ADARB1

Plasmid

#155396

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ADARB1 (ADARB1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Rat, Mouse, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)

Use

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHDM-SARS-CoV-2 Δ18

Plasmid

#164434

Purpose

Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike truncated to remove 18 amino acids (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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