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GoldyTALEN-ppp1cab TAL1

Plasmid

#41835

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

protein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pM-ErbB4-delta-kinase-PY3 mutant

Plasmid

#17802

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Use

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
MFE23-ZZ

Plasmid

#223553

Purpose

MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.

Depositor

Jean Gariepy

Article

Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

Insert

pET302-MFE23-ZZ

Use

Tags

HA tag and HIS tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHThMeCP2 (pc3972)

Plasmid

#227639

Purpose

Mammalian expression vector for human MeCP2 C-terminally tagged to HaloTag.

Depositor

Cristina Cardoso

Article

Schmidt et al Cells. 2024 Jan 11;13(2):139. doi: 10.33

Insert

MeCP2 (MECP2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/V5-His-mPer1

Plasmid

#219421

Purpose

Expresses 3873 bp of mPer1 coding sequence in E. coli.

Depositor

Aziz Sancar

Article

Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mPer1 (Per1 Mouse)

Use

Tags

His and V5

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCKC014

Plasmid

#226623

Purpose

pLenti wt hTdT template for library 3

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

Terminal deoxynucleotidyl transferase (DNTT Human)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MAVS-ts3

Plasmid

#174276

Purpose

MAVS knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MAVS shRNA (MAVS Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHMTc-FBF-2 (121-632)

Plasmid

#218664

Purpose

Expression of MBP:6xHIS:FBF-2(121-632)

Depositor

Judith Kimble

Article

Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

Insert

fbf-2 (fbf-2 Nematode)

Use

Tags

MBP-6xHIS

Expression

Bacterial

Mutation

amino acids 121-632 only

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJK2097

Plasmid

#218665

Purpose

Expression of MBP:6xHIS:FBF-2(Y479A, 121-632)

Depositor

Judith Kimble

Article

Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

Insert

fbf-2 (fbf-2 Nematode)

Use

Tags

MBP-6xHIS

Expression

Bacterial

Mutation

amino acids 121-632 only, with Y479A mutation

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-BS-AtMIR390a-B/c

Plasmid

#199559

Purpose

GATEWAY-compatible entry vector

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2023 Oct 27;51(19):10

Insert

Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pENeCFPCNAL2 pc922

Plasmid

#167563

Purpose

For construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.

Inserts

Proliferating Cell Nuclear Antigen (PCNA Human)
CFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCENPBR pc861

Plasmid

#167569

Purpose

Human CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).

Depositor

Cristina Cardoso

Article

Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

Insert

Homo sapiens centromere protein B (CENPB Human)

Use

Tags

Ds-Red

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMeCP2miRFP670 pc3942

Plasmid

#167568

Purpose

The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.

Depositor

Cristina Cardoso

Article

Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

Insert

MeCp2 (Mecp2 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 Blast CRLF2

Plasmid

#194090

Purpose

Expression of human CRLF2 gene

Depositor

Martin Carroll

Article

Hurtz et al J Clin Invest. 2020 Jul 1;130(7):3637-36

Insert

CRLF2 (CRLF2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pEE047

Plasmid

#176804

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

503

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRD403

Plasmid

#163108

Purpose

Expression of mScarlet-I_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mScarlet-I_H-NSdbd

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSHDY*in_PpetE:BCD2:MrBBS:Strep

Plasmid

#133973

Purpose

Heterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.

Depositor

Pia Lindberg

Article

Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

Insert

MrBBS:StrepII

Use

Synthetic Biology

Tags

StrepII

Expression

Bacterial

Mutation

inserted gene is codon optimized for Synechocyst…

Promoter

PpetE:BCD2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisA-HOXC6-2

Plasmid

#110365

Purpose

Mammalian expression of HisA-tagged HOXC6-2

Depositor

Carlos Moreno

Article

Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

Insert

HOXC6 (HOXC6 Human)

Use

Tags

HIS

Expression

Mammalian

Mutation

short isoform

Promoter

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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563aa ELL2 pEF4B

Plasmid

#127273

Purpose

Expresses 563aa human WT ELL2

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 truncated at SphI (ELL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Contains 564 N-terminal amino acids ELL2, termina…

Promoter

pEF1a

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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