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pCRII-Topo Plexin D1 in situ probe

Plasmid

#45621

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Plexin D1 in situ probe (Plxnd1 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains bp# 5751-6406 of NM_026376.3

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
L0-I18 Ub-Arg GG1-GG2

Plasmid

#195899

Purpose

Level 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Ub-Arg tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBSc polyA + Shal2

Plasmid

#16144

Depositor

Larry Salkoff

Article

Butler et al Science. 1989 Feb 17. 243(4893):943-7.

Insert

Shal (Shal Fly)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
5I3L (DPF3)

Plasmid

#79522

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

DPF3 (DPF3 Human)

Use

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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L1P63

Plasmid

#195938

Purpose

PhiC31-intein(DnaE-Npu) and Intein(DnaE-Ssp)-PhiC31 : pUBQ10-PI-tUBQ1-p35S-IP-t35S

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10-PI-tUBQ1-p35S-IP-t35S

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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L0-I2 pPP2AA3-mutated GG0-GG1

Plasmid

#195891

Purpose

Level 0 pPP2AA3 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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L0-T1 Target PhiC31

Plasmid

#195890

Purpose

Level 0 PhiC31 target: attP_PhiC31-pUBQ10-attB_PhiC31 with golden gate linker in pBLUNT plasmid.

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 Target

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I38 BpiI-pUBQ10-BpiI

Plasmid

#195888

Purpose

Level 0 pUBQ10 promoter between BpiI sites for target construction

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L1P5

Plasmid

#195920

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-DST-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3-PhiC31-NLS-DST-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P19

Plasmid

#195924

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-NLS-DST-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10-PhiC31-NLS-DST-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P24

Plasmid

#195927

Purpose

Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-NLS-DST-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

p35S-PhiC31-NLS-DST-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I9 PhiC31 GG1-GG4

Plasmid

#195892

Purpose

Level 0 PhiC31 integrase with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMXLK_cFq

Plasmid

#114236

Purpose

POC12376, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type c-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Cerulean-tagged Apollo-NADP+

Plasmid

#71800

Purpose

Expresses Cerulean-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

Depositor

Jonathan Rocheleau

Article

Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

Insert

Apollo-NADP+

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-array 8

Plasmid

#175580

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-tagged Apollo-NADP+

Plasmid

#71797

Purpose

Expresses Venus-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

Depositor

Jonathan Rocheleau

Article

Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

Insert

Apollo-NADP+

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-IS186

Plasmid

#175581

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pQCascade(Ptr)-entry(BsaI)

Plasmid

#175579

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-H12TEV-hEIF5B-1~1220(FL)

Plasmid

#208636

Purpose

for E. coli expression of human eIF5B (ENST00000289371)

Depositor

Quanfu Ma

Insert

human eIF5B (ENST00000289371) (EIF5B Human)

Use

Tags

6xhis

Expression

Bacterial

Mutation

Contain SNP rs1376443, protein sequence not chang…

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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T1P/pUBQ10(mTurq->mScarlet)

Plasmid

#195945

Purpose

PhiC31 target with pUBQ10 switching from mTurq to mScarlet

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

tRBCS-mScarlet-attP_PhiC31-pUBQ10-attB_PhiC31-mTurq-tUBQ10

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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