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Plasmid#174459PurposeExpress iRFP with late endosome-targeting sequence in mammalian cellsDepositorInsertiRFP with late endosome-targeting sequence
UseTagssmall GTPase (Rab7)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV+ plasmid
Plasmid#169740PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKH-1699
Plasmid#124227PurposeMammalian SpCas9-HF1 expressionDepositorInsertSpCas9-HF1
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRPL28 SpHIS5 (pNTI663)
Plasmid#115433PurposeExpresses wild-type RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationMfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(Q38P) SpHIS5 (pNTI664)
Plasmid#115434PurposeExpresses Q38P RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationQ38P; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(H39L) SpHIS5 (pNTI665)
Plasmid#115435PurposeExpresses H39L RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationH39L; MfeI site in intronPromoterashbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc
Plasmid#91935PurposeBacterial expression of maize SUMO1a and inactive SCE1dDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc
UseTags6His and MycExpressionBacterialMutationC94S active site mutation and Truncation of ZmSUM…PromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc
Plasmid#91933PurposeBacterial expression of maize SUMO1a and inactive SCE1bDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc
UseTags6His and MycExpressionBacterialMutationC94S active site mutation and Truncation of ZmSUM…PromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)
Plasmid#139448PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-Q76L (CA)
Plasmid#83422PurposeMouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidue 76 from Q to L (Q76L: CAG -> CTG)PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMy-MouseCTCFbiotag-T2A-mOrange
Plasmid#50564PurposeRetroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-termDepositorInsertsAmpicilin
Mouse CTCF Exons
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-STAT3
Plasmid#111934PurposeExpresses human STAT3 fused to GFP in mammalian cellsDepositorInsertSignal transducer and activator of transcription 3 (STAT3 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_G299C
Plasmid#177829PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_G299C
UseTagsExpressionMammalianMutationG299CPromoterCMVAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_A215C
Plasmid#177828PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_A215C
UseTagsExpressionMammalianMutationA215CPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
UseTagsExpressionMammalianMutationA191GPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_GFP_C289T
Plasmid#177826PurposeSite-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).DepositorInsertGFP_C289T
UseTagsExpressionMammalianMutationC289TPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-AT
Plasmid#133306Purpose"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisationDepositorInsertaxe/txe toxin-antitoxin cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Puro_siKD
Plasmid#86695PurposeTetracycline inducible expression of shRNA targeted to AAVS1 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterH1 TOAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F4
Plasmid#55787PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)DepositorInsert3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
DsRed-Rab5 DN
Plasmid#13051DepositorInsertRAB5A (Canis familiaris) (RAB5A Canis familiaris)
UseTagsDsRed2 and HAExpressionMammalianMutationasparagine 3 changed to serine (neutral); aspara…PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-T31N (DN)
Plasmid#83423PurposeMouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidue 31 from T to N (T31N: ACG -> AAC)PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
DNM2 delta PRD
Plasmid#168275PurposeExpress PRD-deleted DNM2 mutant (aa 1-742) in pEGFP-N1 vector.DepositorInsertDNM2 (DNM2 Human)
UseTagsGFPExpressionMammalianMutationaa 1-742PromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-HS
Plasmid#133305Purpose"Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisationDepositorInserthok/sok toxin-antitoxin cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)
Plasmid#139445PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)
Plasmid#139446PurposeLentiviral vector genome to ectopically express Firefly luciferaseDepositorInsertFLAG-tag Firefly luciferase
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-E5TAT-GFP-HiBit
Plasmid#170993PurposeFor bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-E5TAT-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-GFPg1 (BB09)
Plasmid#139461PurposeLentiviral vector with gRNA targeting GFP; includes puromycin selectable markerDepositorInsertGFP-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-CTRLg2
Plasmid#139451PurposeLentiviral vector with non-targeting gRNA and neomycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: neoR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-CTRLg1
Plasmid#139450PurposeLentiviral vector with non-targeting gRNA and neomycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: neoR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-CTRLg1 (CG509)
Plasmid#139455PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-TAT-GFP-HiBit
Plasmid#170991PurposeFor bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-TAT-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F1
Plasmid#55784PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)DepositorInsert3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-Rab21-CCSS
Plasmid#83424PurposeMouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidues 218 and 219 from C to S (CCSS: TGCTGT -&…PromoterCMVAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV6- Entry-RPL26DC-DDK
Plasmid#133867PurposeExpression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sitesDepositorInsertRPL26DC-myc-FLAG (RPL26 Human)
UseTagsMyc-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pAT-9208
Plasmid#124221PurposeCloning plasmid for a self-targeting gRNA libraryDepositorInsertself-targeting gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-HA2-GFP-HiBit
Plasmid#171017PurposeFor bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-HA2-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F3
Plasmid#55786PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)DepositorInsert3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F6
Plasmid#55789PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)DepositorInsert3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only