-
Plasmid#105556PurposeAAV expression of EGFP from tMCK promoterDepositorHas ServiceAAV9InsertEGFP
UseAAVTagsExpressionMammalianMutationPromotertMCKAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27
Plasmid#22902DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK27 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
spNW80
Plasmid#173487PurposeExpresses spNW80 in Bl21DepositorInsertspNW80
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagD 77L
Plasmid#19308DepositorInsertRas-related GTP binding D (RRAGD Human)
UseTagsGST and HAExpressionMammalianMutationS77LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pLV-EF1a-V5-LIC
Plasmid#120247PurposeFor LIC cloning with N-terminal V5 tagDepositorTypeEmpty backboneUseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 3xFLAG TPP1 88-544
Plasmid#53548Purposemammalian expression of TPP1 full lengthDepositorInsertTPP1 (ACD Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-UXPS
Plasmid#172136Purposemammalian expression plasmid for a UDP/UTP sensorDepositorInsertUXPS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-MDC1 (770-7)
Plasmid#26284DepositorInsertMediator of DNA damage checkpoint 1 (MDC1 Human)
UseEntry vectorTagsGFPExpressionMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGRG36
Plasmid#16666DepositorInsertmTn7::MCS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDV044
Plasmid#191824PurposePlasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpRDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp
Plasmid#101349Purpose2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3DepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta tat delta TAR delta env GFP in Nef Tet ope…PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Cas9-2A-GFP-sgRNA
Plasmid#124889PurposeContains spCas9-2A-GFP and guide RNA scaffold in single retroviral vectorDepositorInsertCas9
UseRetroviralTags2A-GFPExpressionMutationPromoterMSCVAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
LT3GECIR
Plasmid#111178PurposeTet-ON miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterT3GAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSA_2_mTagBFP2_synCoTC
Plasmid#199474PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-AznLRS
Plasmid#172482PurposeFor E.Coli expression, 306L309L348I384F411WDepositorInsertAznLRS and pylTcua
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xHA-hTERT
Plasmid#51637PurposeMammalian expression of 3xHA tagged human TERT, N-terminal.DepositorInsertTERT (TERT Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1/PD-L1
Plasmid#121478Purposeexpression of human PD-L1 in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR
Plasmid#191795Purposevector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6DepositorInserthumanized hfCas13d
UseAAV and CRISPRTagsExpressionMammalianMutationA134V, A140V, A141V, A143VPromoterEFS, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_mTagBFP2_synCoTC
Plasmid#199472PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Mito-7
Plasmid#55248PurposeLocalization: Mitochondria, Excitation: 383, Emission: 448DepositorInsertMito (COX8A Human)
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
SpCas9 TadCBEd-V106W
Plasmid#193839PurposeExpress TadCBEd-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd V106W-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CMV::V5-EGFR
Plasmid#199717PurposeExpresses V5-tagged EGFR in mammalian cells.DepositorInsertV5-EGFR
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/td5StayGold(c4)=GianCreg
Plasmid#212022PurposeFor labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].DepositorInsertGianCreg (GOLGB1 Human)
UseTagstd5StayGold(c4)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-EWS-FLI1
Plasmid#102813PurposeOverexpress EWS-FLI1. 3rd generation lentiviral vector.DepositorInsertEWS-FLI1 (EWSR1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRESCA-syntide
Plasmid#132415PurposeFRET-based sensor for CaMKII activityDepositorInsertFRESCA-syntide
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-FLAG-cGAS
Plasmid#127661PurposeOver-express EGFP-FLAG-cGASDepositorInsertcGAS (CGAS Human)
UseLentiviralTagsEGFP-FlagExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC
Plasmid#64874Purposelentiviral vector with Flag, HA tagsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA3.1-HDAC2-H141A
Plasmid#115346PurposeExpresses mouse HDAC2 cDNA with H141A point mutationDepositorInsertHistone deacetylase 2 (Hdac2 Mouse)
UseTagsFLAGExpressionMammalianMutationH141APromoterCMVAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB_iETV2_P2A_GFP_Puro
Plasmid#168805PurposePiggyBac transposon construct with doxycycline inducible ETV2 + GFP expressionDepositorInsertETV2 (ETV2 Synthetic, Human)
UseSynthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GAPDH
Plasmid#83910Purposestable overexpressionDepositorInsertglyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en1FnCas9
Plasmid#201947PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en1FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationE1369R on FnCas9PromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro GFP shRNA
Plasmid#10675DepositorInsertGFP shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
hGli1 flag3x
Plasmid#84922Purposemammalian expression of Gli1DepositorInsertGli1 (GLI1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCE-hSK
Plasmid#41814PurposeNon-integrating (episomal) expression of human SOX2 and KLF4DepositorUseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0884
Plasmid#142702PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXB4 (HOXB4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOS008: GCaMP6F calcium sensor (cytosolic)
Plasmid#163045PurposeGCaMP6F calcium sensor (cytosolic)DepositorInsertGCaMP6F
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCKanR-Mu-BsaI
Plasmid#79769PurposeTransposon-propagation plasmid for Mu-BsaI modified transposonDepositorInsertMu-BsaI
UseTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSH-CAG-AtAFB2.F74A-IRES-puro
Plasmid#216245PurposeExpress AtAFB2.F74A mutant without tag for AID2DepositorInsertAtAFB2.F74A
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsExpressionMammalianMutationF74A mutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only