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Plasmid#192415PurposeTo act as a 1-input state of the split-NAND gate.DepositorInsertFRT-Act2-FRT::RlucRluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP691_L2_2I_FRT-OCS_Rluc_Flp_C1
Plasmid#192414PurposeTo act as the 2-input state of the split-AND gate.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP764_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IB_lacZ
Plasmid#192408PurposeTo act as a 1-input state of the A NIMPLY B gate.DepositorInsertB3RT-Act2-B3RT::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSNRW-Z3-Nluc
Plasmid#174486PurposeRecombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.DepositorInsertZ3-Nluc
UseTags6x Histidine tagExpressionBacterialMutationPromoterT7lac promoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
double_sgRNA targeting e1 and e4
Plasmid#190688PurposesgRNAs targeting enhancer 1 and 4 of MYC separatelyDepositorInsertsgRNAs targeting enhancer 1 and 4 of MYC separately
UseCRISPR, Lentiviral, and Synthetic BiologyTagsBFP and PuromycinExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
rtTA-sg-CMV51
Plasmid#188484PurposegRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.DepositorInsertCMV promoter sgRNA
UseCRISPRTagsExpressionMammalianMutationNonePromoterU6Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
L1-AA027
Plasmid#185752PurposeLevel 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).DepositorInsertHygR2
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2341_Tier3(SB)-Puro
Plasmid#169639PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2403-Tier1-PhCMV-ZeoR
Plasmid#169495PurposeTier-1 vector encoding PhCMV-driven zeocin resistance gene ZeoR (PhCMV-ZeoR-pA).DepositorInsertPCMV-driven Sh ble
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_sgRNA_Ago1_5
Plasmid#172470PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1DepositorInsertsgAgo1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_sgRNA_Ago1_6
Plasmid#172471PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1.DepositorInsertsgAgo1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pb mD3S4-dN
Plasmid#180252PurposeP. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.DepositorInsertD3 of P. berghei HAP2 with N-glycosylation sites mutated
UseTags6HisExpressionMammalianMutationN516T, S533N and N539QPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B S395E
Plasmid#176006PurposeExpresses WIPI2B (S395E) fused to Halo in mammalian cellsDepositorInsertWIPI2B (S395E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationSerine 395 to GlutamatePromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B S395A
Plasmid#176005PurposeExpresses WIPI2B (S395A) fused to Halo in mammalian cellsDepositorInsertWIPI2B (S395A) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationSerine 395 to AlaninePromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJPS2955
Plasmid#135589PurposeSNR6 has UUUAUUUC at position 100–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
UseTagsExpressionYeastMutationU6Δ8 has UUUAUUUC at position 100–107 deletedPromoterNativeAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
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p415:iCas9
Plasmid#138266PurposeExpression of mTN3-GGS6-dCas9 (iCas9) in yeast.DepositorInsertmTN3-GGS6-dCas9
UseCRISPRTagsExpressionYeastMutationCas9-D10A, H840APromoterGalLAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS424-ysg(NT)
Plasmid#138258PurposeExpression of a non-targeting sgRNA to be used as a control in a yeast dual reporter systemDepositorInsertNontargeting sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only