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pCDNA3 Flag BimL(T56D,DE)

Plasmid

#25060

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56D,DE) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid changed le…

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB6 Cterminal tail

Plasmid

#60403

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-Claspin-BPI

Plasmid

#31633

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 273-492 of claspin

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 1-373 pBacPAK8

Plasmid

#15328

Depositor

Ronald Conaway, Joan Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Expression

Mutation

Amino acids 1-373

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 45-621 pBacPAK8

Plasmid

#15329

Depositor

Ronald Conaway, Joan Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Expression

Mutation

Amino acids 45-621

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 45-373 pBacPAK8

Plasmid

#15330

Depositor

Ronald Conaway, Joan Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Expression

Mutation

Amino acids 45-373

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG ELL 500-621 pBacPAK8

Plasmid

#15331

Depositor

Ronald Conaway, Joan Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Expression

Mutation

Amino acids 500-621 only

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
lentiMutB3GALT5catdead-blast

Plasmid

#220869

Purpose

lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

Depositor

Jennifer Kohler

Insert

beta-1,3-galactosyltransferase 5 (B3GALT5 Human)

Use

Lentiviral

Tags

myc, FLAG

Expression

Mammalian

Mutation

encodes D156A and D243A mutations; also, nucleoti…

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-TAPP1(PHD)

Plasmid

#221757

Purpose

Stable expression of GFP-TAPP1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PLEKHA1 (PLEKHA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem TAPP1 (PHD) (amino acids 184-304)

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PKD C1ab

Plasmid

#221755

Purpose

Stable expression of GFP-PKD C1ab in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Prkd1(138-343) (Prkd1 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 138-343

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-cTNT-Cre-miR122 TS

Plasmid

#209784

Purpose

Expresses Cre by the specific cTNT promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

miR122 ts

Use

AAV

Tags

Expression

Mutation

Promoter

cTNT

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-RPT6-FLAG

Plasmid

#205416

Purpose

Expresses dCas9-RPT6 with FLAG label

Depositor

Timothy Jarome

Article

Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

Insert

dCas9-RPT6 (Psmc5 Rat)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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hARL15

Plasmid

#209798

Purpose

Bacterial expression of human ARL15

Depositor

Kalle Gehring

Article

Mahbub et al Elife. 2023 Jul 14;12:e86129. doi: 10.75

Insert

ARL15 (ARL15 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-hARL1(Q71L)

Plasmid

#206407

Purpose

Mammalian expression of hARL1(Q71L)

Depositor

Hye-Won Shin, Kazuhisa Nakayama

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q71L

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-hARL1(T31N)

Plasmid

#206408

Purpose

Mammalian expression of hARL1(T31N)

Depositor

Hye-Won Shin, Kazuhisa Nakayama

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T31N

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW pHluorin-SYT9 IRES mRuby3

Plasmid

#195698

Purpose

Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt9 IRES mRuby3

Plasmid

#195700

Purpose

Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

encodes only the first 68 amino acids of Tac1

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2B D330, 332N

Plasmid

#195705

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9 with D3…

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2AB D197,199,330,332N

Plasmid

#195706

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 104-386 of mouse SYT9 with D1…

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHD22Y/PfPRMT5 deletion

Plasmid

#203161

Purpose

for disruption of PfPRMT5 gene

Depositor

Liwang Cui

Article

Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

Insert

PfPRMT5 fragment

Use

Tags

3 myc tags and 3' UTR of P. berghei dihydrof…

Expression

Mammalian

Mutation

Promoter

No

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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