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pPBpuro-mNG-eDHFR(69K6)-p85iSH2

Plasmid

#172103

Purpose

Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFOSGE_DUM1H01

Plasmid

#71090

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

nej (nej Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
CD58-bio-His

Plasmid

#53153

Purpose

Expresses full-length CD58 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

CD58 (CD58 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pSpCAS9n(BB)-2A-puro DNM1, A

Plasmid

#107795

Purpose

gRNA vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

gRNA targeting human DNM1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCAS9n(BB)-2A-puro DNM1, B

Plasmid

#107796

Purpose

gRNA vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

gRNA targeting human DNM1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3-K24,83,88,104A

Plasmid

#58408

Purpose

Expresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, 88, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Flag-mRIPK1-S321E

Plasmid

#115340

Purpose

Mammalian expression of Flag-mRIPK1-S321E

Depositor

Junying Yuan

Article

Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

Insert

RIPK1 (Ripk1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

S321E

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Clavesin1 (pEGFP-C1-Sec14a)

Plasmid

#47421

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin1 (CLVS1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
ATF4 17: 5’ATF4.uORF1stopm1&2.GFP

Plasmid

#21863

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stop codons between the colinear uORF1 and uORF2 …

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro NM23 H1 His

Plasmid

#11365

Depositor

Ronald Kahn

Article

Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

NM23 H1 (NME1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

His118 mutation to Phe (abolishes histidine kinas…

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
pRK-HA-TRAF4

Plasmid

#58314

Purpose

HA-tagged mouse TRAF4

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pPFAS-2XStrep

Plasmid

#125682

Purpose

expresses human PFAS protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PFAS (PFAS Human)

Use

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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WNT11-bio-His

Plasmid

#53430

Purpose

Expresses full-length Protein Wnt-11 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

WNT11 (WNT11 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pUCHR-CD5Flag2-P2A-smAID-mClover

Plasmid

#110657

Purpose

Lentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degron

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CD5Flag2-P2A-AID-mClover

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBSSK p85 alpha (delta SH3, iSH2)

Plasmid

#13433

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta SH3, iSH2) (PIK3R1 Human)

Use

Tags

Flag

Expression

Bacterial

Mutation

Flag tags replacing both the SH3 and iSH2 domains.

Promoter

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3-deltaY20

Plasmid

#58459

Purpose

Expresses murine IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 deleted

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/NS5-Af-mut

Plasmid

#120829

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

ZIKV NS5 V114M/V872M - "african mut"

Use

Tags

V5

Expression

Mammalian

Mutation

V114M/V872M

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDN-NG1Tt

Plasmid

#44523

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1 (wt) promoter (GAL1 Budding Yeast)
tetR

Use

Synthetic Biology; Expression regulator/reporter

Tags

Expression

Yeast

Mutation

Promoter

PGal1 (wt)

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mIFITM3-Y20A

Plasmid

#58460

Purpose

Expresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 to alanine

Promoter

CMV IE

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
pJZC39

Plasmid

#62333

Purpose

sgRNA + 1x PP7 with mCherry for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x PP7
mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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