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HA.PKD.S738E/S742E

Plasmid

#10810

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Activation loop active: S738E and S742E (bp 2212-…

Promoter

CMV

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pRcCMV myc-Plk1 K82R (Nigg LA184)

Plasmid

#41157

Depositor

Erich Nigg

Article

Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

Insert

Plk1 (PLK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

K82R

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
Cdk5-DN

Plasmid

#1871

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk5 (CDK5 Human)

Use

Tags

Expression

Mammalian

Mutation

D145N, dominant negative

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
CAG-mCherry-Otx2shRNA1

Plasmid

#73979

Purpose

Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Otx2 gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB6638

Plasmid

#106165

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB6364

Plasmid

#106151

Purpose

EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

Cas9

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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Twin-Strep(R)-Tev-GSGS-Ubiquitin

Plasmid

#186803

Purpose

Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

Twin-Strep(R)-Tev-GSGS-Ubiquitin

Use

Tags

GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…

Expression

Bacterial

Mutation

Strep tag Lysine's mutated to Arginine

Promoter

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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ALIX-His(1-868, P801G)

Plasmid

#186808

Purpose

Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

ALIX-His (1-868, P801G) (PDCD6IP Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Proline 801 to Glycine

Promoter

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV mouse Dscam

Plasmid

#18737

Depositor

Joshua Sanes

Article

Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

Insert

Dscam (Dscam Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCfB9337 (pgRNA_IV-1_NatMX)

Plasmid

#161590

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAJAK-71 (pESC-NatMXsyn-USER)

Plasmid

#78232

Purpose

Empty vector, for the insertion of gRNA expression cassettes into the vector.

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (1-575)

Plasmid

#1318

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (1-575) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa1-575 of Hsp104

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-CIBN-CreC

Plasmid

#75267

Purpose

The first half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

N-terminal portion of the CIB1 transcription factor

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGRCRE

Plasmid

#129116

Purpose

Expresses cre recombinase in Z. rouxii yeast cells

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Insert

cre

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

S. cerevisiae GAL1 promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET3d::strep-AID

Plasmid

#15924

Depositor

Nina Papavasiliou

Article

Dickerson et al J Exp Med. 2003 May 19. 197(10):1291-6.

Insert

AID (AICDA Human)

Use

Tags

strep

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pCfB6627

Plasmid

#106159

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Cdk2-DN

Plasmid

#1882

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk2 (CDK2 Human)

Use

Tags

Expression

Mammalian

Mutation

D146N, dominant negative

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
HA-RNF34

Plasmid

#119938

Purpose

RING finger protein 34 expression (HA-tag at the N-terminus)

Depositor

Angel de Blas

Article

Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

Insert

RNF34 (Rnf34 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA-Mirk (YF)

Plasmid

#17845

Depositor

Eileen Friedman

Article

Lee et al Cancer Res. 2000 Jul 1. 60(13):3631-7.

Insert

Mirk (DYRK1B Human)

Use

Tags

6xHis and Anti-XPress

Expression

Mammalian

Mutation

Y271/273F

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-attBr-ZsGreen-attP

Plasmid

#51265

Purpose

Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

attBr-ZsGreen-attP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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