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pCMV2-hNCOA6:V5

Plasmid

#63585

Purpose

A subunit of the Trithorax-related (Trr) methyltransferase complex.

Depositor

Kenneth Irvine

Article

Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

Insert

NCOA6 (NCOA6 Human)

Use

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pTK_BsmBI_LacZ_Cerulean_Nanotag_60

Plasmid

#130528

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo5

Plasmid

#157804

Purpose

Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo5

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cherry_Nanotag_117

Plasmid

#130552

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_91

Plasmid

#130539

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_23_neg_Control

Plasmid

#130562

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-RvLEAM

Plasmid

#90035

Purpose

To express GFP-tagged RvLEAM in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro

Plasmid

#174119

Purpose

Expression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

sgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mU6 and UbC

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pES.HSV2.3a

Plasmid

#69593

Purpose

TCR a chain construct for generation of gBT-I mice

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha chain cDNA from clone HSV2.3 (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

H-2Kb

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only
p3A9cbetaTCR.HSV2.3

Plasmid

#69594

Purpose

TCR beta chain genomic transgenic construct for generation of gBT-I mice

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR beta VJ region from clone HSV2.3 (Tcrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pTK_Citrine_Nanotag_5_neg_Control

Plasmid

#130558

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27 RNAi-resistant

Plasmid

#163620

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 RNAi-resistant (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

RNAi resistance

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

Plasmid

#164266

Purpose

sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6 promoter from W05B2.8

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_24_positive_Control

Plasmid

#130570

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC1

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcry:GFP,-503unc:hACTA1GFP

Plasmid

#172529

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 503bp unc-45b muscle-specific promoter drives expression of GFP-tagged human ACTA1.

Depositor

Joachim Berger

Article

Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:

Insert

ATCA1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCRII-HSV2.3 TCR beta chain

Plasmid

#74077

Purpose

gBT-I TCR beta chain cDNA in pCRII

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR beta cDNA clone HSV2.3 (Tcrb Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMA4.1 (mouse integrin alpha 4 probe)

Plasmid

#14098

Depositor

Richard Hynes

Article

Yang et al Development. 1995 Feb . 121(2):549-60.

Insert

Mouse Alpha 4 Integrin (Itga4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Lin28BdelN70

Plasmid

#51374

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

Lin28B (LIN28B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 70 amino acids deletion

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pTK_Cerulean_Nanotag_70_neg_Control

Plasmid

#130568

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Cerulean_Nanotag_66_neg_Control

Plasmid

#130567

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge