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Plasmid#189846PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.DepositorTypeEmpty backboneUseCre/LoxTagsmRFP and tagBFPExpressionMutationPromoterbeta ActinAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only
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Lyn-FLINC-EKAR1(TA)
Plasmid#87708PurposeNegative-control mutant of Lyn-FLINC-EKAR1.DepositorInsertLyn-FLINC-EKAR1(TA)
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP2.2
Plasmid#189842PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.DepositorTypeEmpty backboneUseCre/LoxTagsGFP GM2 variant and mRFPExpressionMutationPromoterbeta ActinAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP2.3
Plasmid#189843PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.DepositorTypeEmpty backboneUseCre/LoxTagsGFP GM2 variant and mRFPExpressionMutationPromoterbeta ActinAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP8.3
Plasmid#189848PurposeIntron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.DepositorTypeEmpty backboneUseCre/LoxTagsmRFP and tagBFPExpressionMutationPromoterbeta ActinAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLINC-AKAR1(TA)-CAAX
Plasmid#87706PurposeNegative-control mutant of FLINC-AKAR1-CAAX.DepositorInsertFLINC-AKAR1(TA)-CAAX
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMMACE DEST CMV PE2
Plasmid#206276PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertPE2
UseCRISPR; Recombinant baculovirus production, multi…TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1012 - pAAV EF1a DIO Nuc-eYFP
Plasmid#75082PurposeAn AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.DepositorInsertNuc-eYFP
UseAAV and Cre/LoxTagsNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC710 - pAAV EF1a DIO Mem-AcGFP
Plasmid#75081PurposeAn AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.DepositorInsertMem-AcGFP
UseAAV and Cre/LoxTagsPalmitylation siteExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAP1
Plasmid#63674PurposeAccepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.DepositorInsertRFP cloning selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only