Addgene

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pcDNA3.1+HIF1a-AS1_deltaExon2

Plasmid

#194173

Purpose

Expresses lncRNA HIF1a-AS1 Exon1

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Exon2 was deleted

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_deltaExon1

Plasmid

#194174

Purpose

Expresses lncRNA HIF1a-AS1 Exon2

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Exon1 was deleted

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)

Plasmid

#194175

Purpose

Expresses lncRNA HIF1a-AS1 mutant

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Within Exon1, nucleotides 26-78 were removed

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_1200_unspliced

Plasmid

#194176

Purpose

Expresses first 1200 nt of lncRNA HIF1a-AS1 (unspliced)

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-W80A-10xHis

Plasmid

#194179

Purpose

Expresses MPP8-W80A_10xHis

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-W80A inside chromo domain

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta55-118-10xHis

Plasmid

#194180

Purpose

Expresses MPP8-delta55-118_10xHis

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta55-118 (chromo domain deletion)

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta2-441-10xHis

Plasmid

#194181

Purpose

Expresses MPP8-delta2-441_10xHis

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta2-441

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta442-860-10xHis

Plasmid

#194182

Purpose

Expresses MPP8-delta442-860_10xHis

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta442-860

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta600-728-10xHis

Plasmid

#194183

Purpose

Expresses MPP8-delta600-728_10xHis

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta600-728 (4xANK deletion)

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-YTH-mCherry-Cry2

Plasmid

#177126

Purpose

Express YTH domain fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (YTH domain) (YTHDC1 Human)

Use

Tags

mCherry-CRY2

Expression

Mammalian

Mutation

YTH domain only

Promoter

SFFV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTXBI-mclover-YTHDC1-FL

Plasmid

#177121

Purpose

Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (YTHDC1 Human)

Use

Tags

Mxe GyrA intein-Chitin binding domain for protein…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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83_pAAV-ProC14-CatCh-GFP-WPRE

Plasmid

#125949

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC14

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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82_pAAV-ProC13-CatCh-GFP-WPRE

Plasmid

#125948

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC13

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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VII_pAAV-ProB8-GFP-WPRE

Plasmid

#125928

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProB8

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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IV_pAAV-ProB6-CatCh-GFP-WPRE

Plasmid

#125926

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProB6

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
II_pAAV-ProB5-CatCh-GFP-WPRE

Plasmid

#125925

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProB5

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
76_pAAV-ProA25-CatCh-GFP-WPRE

Plasmid

#125908

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA25

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
59_pAAV-ProA16-CatCh-GFP-WPRE

Plasmid

#125899

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA16

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
28_pAAV-ProA10-CatCh-GFP-WPRE

Plasmid

#125894

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA10

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
6_pAAV-ProA37-CatCh-GFP-WPRE

Plasmid

#125920

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA37

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge