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pAAV-sL7-Cre-HA-WPRE

Plasmid

#204488

Purpose

For production of driver AAV virus for Purkinje cell-specific expression

Depositor

Samuel Wang

Article

Chen et al Nature. 2022 May;605(7911):722-727. doi:

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

sL7

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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VI_pAAV-ProA7-GFP-WPRE

Plasmid

#125891

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA7

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-mDlx-jGCaMP8f-WPRE

Plasmid

#176753

Purpose

jGCaMP8f expression in GABA-ergic interneurons under the control of mDlx enhancer

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

mDlx

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-JEDI-2P-Kv-WPRE

Plasmid

#179465

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P-Kv

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mCherry-C1-WPRE

Plasmid

#159956

Purpose

untargeted marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mCherry-C1

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Agmat.1-GCaMP7f-WPRE

Plasmid

#211492

Purpose

expression of GCaMP7f under artificial promoter Agmat

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GCaMP7f

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-tdTomato-WPRE

Plasmid

#104105

Purpose

Expresses tdTomato under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al Elife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-EYFP-WPRE

Plasmid

#104104

Purpose

Expresses EYFP under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al Elife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-JEDI-1P-WPRE

Plasmid

#202616

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAP

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GFAP

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-JEDI-2P-WPRE

Plasmid

#179466

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-jGCaMP8m-WPRE

Plasmid

#176757

Purpose

AAV-mediated expression of GCaMP8m under the EF1a promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-EGFP/CMV/WPREdU3

Plasmid

#17579

Depositor

Jakob Reiser

Article

Ricks et al Stem Cells Dev. 2008 Jun . 17(3):441-50.

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-synaptoPAC-minWPRE

Plasmid

#153085

Purpose

Expresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-mRuby3-WPRE

Plasmid

#107744

Purpose

Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

Depositor

Rylan Larsen

Article

Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

Insert

mRuby3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-2A-oG-WPRE

Plasmid

#102984

Purpose

Lentivirus expressing eGFP and optimised Rabies Glycoprotein (oG)

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

Enhanced Green Fluorescent Protein (eGFP)
Optimised Rabies glycoprotein (oG)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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FCK-CaMKII-Solaris-WPRE

Plasmid

#217480

Purpose

Negative response-polarity hybrid voltage indicator

Depositor

Peng Zou

Insert

Solaris

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cepheid1b-ST-WPRE

Plasmid

#214967

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

Depositor

Peng Zou

Article

Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

Insert

Cepheid1b-ST

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-EFS-MCS-WPRE

Plasmid

#163362

Purpose

The empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectors

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-HaloTag-WPRE

Plasmid

#104108

Purpose

Expresses HaloTag under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al Elife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

HaloTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a CHR2 mCherry WPRE

Plasmid

#129432

Purpose

expression of CHR2 mCherry from EF1a promoter

Depositor

Oskar Laur

Insert

CHR2 mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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