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Plasmid#109139PurposeGFP-tagged CYFIP1 expression constructDepositorInsertCYFIP1 (CYFIP1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9-Cdt1
Plasmid#80426PurposeUsed for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.DepositorInsertSpCas9-Cdt1
UseCRISPRTagsCdt1ExpressionMutationcodon optimized for humanPromoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP2.GFP
Plasmid#109140PurposeGFP-tagged CYFIP2 expression constructDepositorInsertCYFIP2 (CYFIP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLNCX1 myr Akt1 delta4-129
Plasmid#15989DepositorInsertAkt1 (AKT1 Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationDelta 4-129PromoterAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
myc-AP4 Epsilon (V454X)
Plasmid#127624PurposeN-terminal myc-tagged AP4 epsilon subunit (V454X)DepositorInsertAP-4 complex subunit epsilon-1 (AP4E1 Human)
UseTagsmycExpressionMammalianMutationPremature stop codon (V454X)PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Miro1 (1-177 fused to 562-618)
Plasmid#127627PurposeFirst GTPase domain of human Miro1 fused to C-terminusDepositorInsertMiro1 (RHOT1 Human)
UseTagseGFPExpressionMammalianMutation1-177 fused to 562-618PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Miro1 (412-618)
Plasmid#127628PurposeGTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acidsDepositorInsertMiro1 (RHOT1 Human)
UseTagseGFPExpressionMammalianMutation412-618 onlyPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K11R
Plasmid#121154PurposeMammalian expression of HA tagged ubiquitin K11RDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationK11 mutated to an RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9-Gem
Plasmid#80424PurposeUsed for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joiningDepositorInsertSpCas9-Gem
UseCRISPRTagsGemininExpressionMutationcodon optimized for humanPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27R
Plasmid#121155PurposeMammalian expression of HA tagged ubiquitin K27RDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationK27 mutated to an RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K6
Plasmid#121151PurposeMammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationAll Ks apart from K6 mutated to RsPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K6R
Plasmid#121153PurposeMammalian expression of HA tagged ubiquitin K6RDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationK6 mutated to an RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27R-K29R
Plasmid#121156PurposeMammalian expression of HA tagged ubiquitin K27R and K29RDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationK27 and K29 mutated to RsPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K11
Plasmid#121152PurposeMammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationAll Ks apart from K11 mutated to RsPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-Gs protein, alpha subunit [N192/12]
Plasmid#190302PurposeMammalian Expression Plasmid of anti-Gs protein, alpha subunit (Cattle). Derived from hybridoma N192/12.DepositorInsertanti-Gs protein, alpha subunit (Bos taurus) recombinant Mouse monoclonal antibody (GNAS Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pP150
Plasmid#113201Purposeshuttle vector, N-terminal mTurquoise2 taggingDepositorTypeEmpty backboneUseDictyostelium discoideum shuttle vector, n-termin…TagsmTurquoise2ExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
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