-
Plasmid#28167PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRAB GDP dissociation inhibitor (PFL2060c Plasmodium falciparum)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-CDPK3
Plasmid#28151PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCDPK3
UseTagsHis tag with TEV cleavageExpressionBacterialMutationCAD domain (residues 425-602).PromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
ATX3-77Q
Plasmid#184249PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertAtaxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Synthetic, Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationCodon optimization for protein expression in BL2…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3-77Q-R388G
Plasmid#184251PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Synthetic, Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationC-terminal codon optimization for protein expres…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3 13Q- I77K Q78K W87K
Plasmid#185908PurposeExpresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).DepositorInsertataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Synthetic, Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationPoint mutations in Atx3 gene I77K Q78K and W87KPromoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
438-GST
Plasmid#97092PurposeGST-tev-yORFDepositorTypeEmpty backboneUseTagsGST tag and TEV cleavage siteExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDRD3 (3PMT)
Plasmid#28274DepositorInsertTudor domain-containing protein 3 (TDRD3 Human)
UseTagsHis with TEV cleavageExpressionBacterialMutationcontains Tudor domain, residues 553-611.PromoterAvailable sinceApril 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFHMSP-LIC-N
Plasmid#26095PurposeDonor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expressionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis and TEV cleavage siteExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 15, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
Pf-GMPS
Plasmid#36899PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPf-GMPS (PF10_0123 Plasmodium falciparum)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
5C8H
Plasmid#69969PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertORC2:JMC095-D11:C231601 (ORC2 Human)
UseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-MAPK1 (3OZ6)
Plasmid#28164PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertmitogen-activated protein kinase 1 (cgd2_1960 Cryptosporidium parvum)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains residues 4-390PromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-CDPK1
Plasmid#25280PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertcgd5_820:D42-E517 (cgd5_820 Cryptosporidium parvum)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationContains amino acids D42-E517.PromoterAvailable sinceFeb. 2, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX4T-1_WT_OCT4
Plasmid#40633DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV cleavage, and Thrombin cleavageExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-36α
Plasmid#214343PurposeProtein overexpression in bacteriaDepositorInsertIL36A (IL36A Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_OCT4_T234A_S235A
Plasmid#40634DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV cleavage, and Thrombin cleavageExpressionMammalianMutationT234A_S235APromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
L3MBTL3 (3UT1)
Plasmid#36901PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertL3MBTL3 (L3MBTL3 Human)
UseTagsHis tag and Streptavidin-Binding Peptide (SBP)-Ta…ExpressionBacterialMutationcontains amino acid residues 228-551 onlyPromoterAvailable sinceJune 21, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJL1_CSL3
Plasmid#200173PurposeAmino acids 1-195 of CSL3 with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vectorDepositorInsertCSL3
UseTags6x His tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
RAB3B (3DZ8)
Plasmid#32879PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRAB3B (RAB3B Human)
UseTagsHis tag (with TEV cleavage)ExpressionBacterialMutationamino acids 18-190 onlyPromoterAvailable sinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
SETD3
Plasmid#32873PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETD3 (SETD3 Human)
UseTagsHis tag (with TEV cleavage)ExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits