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Plasmid#41071DepositorInsertTetherin (BST2 Human)
UseTagsFLAGExpressionMammalianMutation74S, 84SPromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1
Plasmid#205443Purposehuman AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55DepositorInserthCD46-HLA-G SCT-hCD47-hCD59
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-MCS-IRES-eGFP-P2A-iCas9
Plasmid#178533PurposeFor genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cellsDepositorInsertiCaspase9 (CASP9 Human)
UseTagsExpressionMammalianMutationFKBP12 F36 to V Truncated Caspase9 (135-416; lack…PromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tet-Inducible Mouse C325A Caspase-9 Mutant
Plasmid#194500PurposeInducible Expression of Mouse C325A Caspase-9 MutantDepositorInsertMouse Caspase-9 C325A Mutant (Casp9 Mouse)
UseTagsNoneExpressionMammalianMutationChanged Cysteine 325 to Alanine to eliminate prot…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-Inducible Human Caspase-9 Alpha C287A Mutant
Plasmid#194709PurposeInducible Expression of Human Caspase-9 Alpha C287A MutantDepositorInsertHuman Caspase-9 C287A Mutant (CASP9 Human)
UseTagsNoneExpressionMammalianMutationChanged Cysteine 287 to Alanine to eliminate prot…PromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible 3xFlag Mouse C325A Caspase-9 Mutant
Plasmid#194501PurposeInducible Expression of N-Terminal 3X FLAG Mouse Caspase-9 C325A MutantDepositorInsertCaspase-9 C325A Mutant (Casp9 Mouse)
UseTags3X FlagExpressionMammalianMutationChanged Cysteine 325 to Alanine to eliminate prot…PromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TRAP1_WT_V5
Plasmid#82994PurposeGateway Donor vector containing TRAP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertTRAP1 (TRAP1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11a_3xFlag-nsp10 (SARS-CoV-2)
Plasmid#169157PurposeTo express SARS-CoV-2 nsp10 in E. coliDepositorInsert3xFlag-nsp5CS[VRLQ]-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
UseTags3xFlag and nsp5CS[VRLQ]: predicted nsp5 protease …ExpressionBacterialMutationCodon optimised for E. coliPromoterT7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible 3xFlag Mouse Caspase-9 ΔLS
Plasmid#194609PurposeInducible Expression of N-Terminal 3X Flag Mouse Caspase-9 Minus Large Subunit DomainDepositorInsertN-Terminal 3X Flag Mouse Caspase-9 Minus Sequence Encoding Val 177 to Asp 353 (Casp9 Mouse)
UseTags3X FlagExpressionMammalianMutationDeletion of Large Subunit Domain eliminates bindi…PromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD
Plasmid#194608PurposeInducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus CARD DomainDepositorInsertN-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus Sequence Encoding Met 1 to Lys 176 (Casp9 Mouse)
UseTags3X FlagExpressionMammalianMutationDeletion of N-terminal CARD domain and changed Cy…PromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible 3xFlag Mouse Caspase-9 ΔB LS
Plasmid#194927PurposeInducible Expression of N-Terminal 3X Flag Mouse Caspase-9 Minus Large Subunit Region Ala 264 to Asp 353DepositorInsertN-Terminal 3X Flag Mouse Caspase-9 Minus Sequence Encoding Ala 264 to Asp 353 (Casp9 Mouse)
UseTags3X FlagExpressionMammalianMutationDeletion of Large Subunit Region Ala 264 to Asp 3…PromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2iApowriter2(#336)
Plasmid#184104Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesisTagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22Apowriter2(#340)
Plasmid#184105Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)Tagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22iuasvApoWtEos3N(#486)
Plasmid#184107Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22iuasvApoWChe(#488)
Plasmid#184108Purposecyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
GRP94-COMP-blac-flag-his
Plasmid#111008PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertendoplasmin, putative (GRP94) (PFL1070c Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔA LS
Plasmid#194926PurposeInducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Large Subunit Region Val 177 to Arg 263DepositorInsertN-Terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Val 177 to Arg 263 (Casp9 Mouse)
UseTags3X FlagExpressionMammalianMutationChanged Cysteine 325 to Alanine to Eliminate Prot…PromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔLD + ΔSS
Plasmid#194610PurposeInducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Linker + Small Subunit DomainDepositorInsertN-terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Ala 354 to Ser 454 (Casp9 Mouse)
UseTags3X FlagExpressionMammalianMutationChanged Cysteine 325 to Alanine to eliminate prot…PromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2iU6Apowriter2N(#355)
Plasmid#184106Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesisDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesis + in vitro trans…Tagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-Ubi+SP6Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only