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Plasmid#200027PurposeLentiviral vector for expression of KSHV ORF29 with D476A mutationDepositorInsertORF29
UseLentiviralTagsExpressionMutationAspartic acid 476 changed to AlaninePromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD2604
Plasmid#175088PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationS644APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2602
Plasmid#175086PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationT424A, T438APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2603
Plasmid#175087PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationT504A, T508APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-ORF30-2xStrep
Plasmid#130958PurposeLentiviral vector for expression of KSHV ORF30 with a C-terminal 2xStrep tagDepositorInsertORF30 (ORF30 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseLentiviralTags2xStrepExpressionMutationPromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-ORF24-3xFLAG
Plasmid#130959PurposeLentiviral vector for expression of KSHV ORF24 with a C-terminal 3xFLAG tagDepositorInsertORF24 (ORF24 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseLentiviralTags3xFLAGExpressionMutationPromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIMVAu1
Plasmid#98301PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.DepositorInsertHMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIMVAd39T
Plasmid#98303PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.DepositorInsertpdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-NHASt-codoptUS2
Plasmid#135102PurposeCodon-optimized HSV-1 US2 with N-terminal HAStrep tagDepositorInsertHSV-1 US2 codon optimized
UseTagsHA-Strep-StrepExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66-2xStrep
Plasmid#130953PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length)PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-NHASt-codoptUL50
Plasmid#135101PurposeCodon-optimized HSV-1 UL50 with N-terminal HAStrep tagDepositorInsertHSV-1 UL50 codon optimized
UseTagsHA-Strep-StrepExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-UL29-egfp-U3-UL8
Plasmid#166694PurposeTo express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcUW51-gEgIL
Plasmid#11622DepositorInsertHSV-1 glycoprotein E and HSV-1 glycoprotein I
UseTagsHisExpressionInsectMutationDicistronic vector containing HSV-1 gE (KOS straiā¦PromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-NHASt-codoptUL23
Plasmid#135104PurposeCodon-optimized HSV-1 UL23 with N-terminal HAStrep tagDepositorInsertHSV-1 UL23 codon optimized
UseTagsHA-Strep-Strep (N terminal on backbone)ExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-codoptUL23-CStHA
Plasmid#135099PurposeCodon-optimized HSV-1 UL23 with C-terminal StrepHA tagDepositorInsertHSV-1 UL23 codon optimized
UseTagsStrep-Strep-HAExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
P63-sp64 Poly A
Plasmid#59435Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 63
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
P29-sp64 Poly A
Plasmid#59434Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 29
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
P21-sp64 Poly A
Plasmid#59433Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 21
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDD2601
Plasmid#175085PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions S47A, S65A, T75A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationS47A, S65A, T75APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-UL29-U3-UL8
Plasmid#166685PurposeTo express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only