Addgene

Showing: 281 - 300 of 15978 results

Results per page:

Sort results by:

pSBtet-GP

Plasmid

#60495

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

TCE & RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacPuroR-NeoR

Plasmid

#34921

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

Inserts

Bacterial promoter::PuroR::bacterial 3p flank
Bacterial promoter::NeoR::bacterial 3p flank

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV PIG (Puro IRES GFP empty vector)

Plasmid

#21654

Purpose

Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pPL5071_TEF1*-Cre_URA3

Plasmid

#60930

Purpose

Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Use

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Mutation

Promoter

mutant TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIH1-puro-control shRNA

Plasmid

#26597

Purpose

Lentiviral control shRNA vector with puromycin selection.

Depositor

Frank Sinicrope

Article

Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-BP

Plasmid

#60512

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

Plasmid

#81251

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA3211

Plasmid

#46879

Purpose

Lentiviral vector for Tet-inducible expression. Puromycin-resistance

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE-Tight

Available since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pPL5608_TEF1*-Cre_TRP1

Plasmid

#60931

Purpose

Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Use

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Mutation

Promoter

Mutant TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Launchpad (AVA2590)

Plasmid

#85442

Purpose

Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

Depositor

Joan Steitz

Article

Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

Insert

FRT-flanked EF1alfa-GFP PGK-Puromycin cassette

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only
pPL5628_TEF1*-Cre_MET15

Plasmid

#60933

Purpose

Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Use

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Mutation

Promoter

mutant TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

Plasmid

#81255

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB37

Plasmid

#72869

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pERB122

Plasmid

#58282

Purpose

const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

FKBP-targeted miRNA
GFP
Puromycin resistance
inducible mCherry-FRB

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p7SKU6SBpur

Plasmid

#160329

Purpose

dual sh RNA vector expressing cfSBFP2 and a puromycin selection marker

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Type

Empty backbone

Use

Tags

cfSBFP2

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-U6insert-EF1puro

Plasmid

#104537

Purpose

A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Article

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-EGFP

Plasmid

#19319

Purpose

3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA3442

Plasmid

#184852

Purpose

Retroviral vector encoding puromycin resistance and Cre recombinase

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

Cre

Use

Cre/Lox and Retroviral

Tags

Myc-tag

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-puroeGFP

Plasmid

#177922

Purpose

Constitutively expresses eGFP with puromycin selection

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rescue plasmid

Plasmid

#135971

Purpose

expresses copGFP and puromycin resistance genes

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge