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pLN429 (FuGW-G8p-mKate2)

Plasmid

#105185

Purpose

Module 3 - GAL4 synthetic promoter ( with eight binding sites) drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-mKate2

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLN593 (FuGW-S(MAFK)p-mKate2)

Plasmid

#105205

Purpose

synthetic promoter MAFK drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(MAFK)p-mKate2

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHIE046 ZF43x12-C mKate2 (TUPV1)

Plasmid

#138726

Purpose

mMoClo TUPV, with ZF43x12-C promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

ZF43x12-Compact

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-2xML1N(7Q)

Plasmid

#67666

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1Nmut*2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pME mKate2-dynactin1-811

Plasmid

#105971

Purpose

entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

dynactin1-811 (DCTN1 Human)

Use

Tags

mKate2

Expression

Bacterial

Mutation

contains only amino acids 1-811

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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MmCavin4-APEX2-P2A-mKate2

Plasmid

#170524

Purpose

Vector for expression of MmCavin4-APEX2-P2A-mKate2 in mammalian cells. Parton lab clone EJF

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Cavin4 (Cavin4 Mouse)

Use

Tags

APEX2-P2A-mKate2

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pME-nCas9n-P2A-mKate2-pA

Plasmid

#109547

Purpose

Multisite gateway vector for expression of nCas9n with bicistronic expression of mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nCas9n and mKate2

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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hsp70: mKate2 dynactin1-811

Plasmid

#105970

Purpose

heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

dynactin1-811 (DCTN1 Human)

Use

Tags

mKate2

Expression

Bacterial

Mutation

contains only amino acids 1-811

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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ath5:mKate2-aPKC-CAAX

Plasmid

#105957

Purpose

transgenesis

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

aPKC CAAX

Use

Tags

mKate2

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLN426 (FuGW-G5p-mKate2)

Plasmid

#105183

Purpose

Module 3 - GAL4 synthetic promoter (with five binding sites) drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G5p-mKate2

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mKate2

Plasmid

#220599

Purpose

Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gγ1-LSS-mKATE2(N)

Plasmid

#207159

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNA

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNG1

Use

Tags

Expression

Mammalian

Mutation

N-terminally tagged with LSS-mKATE2

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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plex_307-mKate-ccdB-zeo

Plasmid

#201126

Purpose

pLEX_307 plasmid with N-terminal mKate tag and zeo marker

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Tags

mKate

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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plex_307-mKate-ccdB-g418

Plasmid

#201125

Purpose

pLEX_307 plasmid with N-terminal mKate tag and g418 marker

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Tags

mKate

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCav1 in pT3TS-Dest

Plasmid

#194293

Purpose

In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin (cav1.S Frog)

Use

In vitro transcription of mrna

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1b in pT3TS-Dest

Plasmid

#194295

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

In vitro transcription of mrna

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1a in pT3TS-Dest

Plasmid

#194294

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1a (cavin1a Zebrafish)

Use

In vitro transcription of mrna

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHIE043 ZF43x1 mKate2 (TUPV1)

Plasmid

#138723

Purpose

mMoClo TUPV, with ZF43x1 promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x1

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

ZF43x1

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE048 ZF43x6-S mKate2 (TUPV1)

Plasmid

#138728

Purpose

mMoClo TUPV, with ZF43x6-S promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

ZF43x6-Spaced

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE047 ZF43x3-S mKate2 (TUPV1)

Plasmid

#138727

Purpose

mMoClo TUPV, with ZF43x3-S promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

ZF43x3-Spaced

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge