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Addgene
Showing: 281 - 300 of 429 results

  1. pBiex PKC - Peptide 14 mCit mCer

    Plasmid
    #83565
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Protā€¦
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Availability
    Academic Institutions and Nonprofits only
    83565-plasmid-map-sequence-id-159510

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  2. ROSA26(MultiFPsĪ”Puro)

    Plasmid
    #140759
    Purpose
    Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).
    Depositor
    Hideyuki Okano
    Article
    Serizawa et al Development. 2019 Nov 4;146(21). pii: de
    Inserts
    puromycin-N-acetyltransferase
    Venus
    mCherry
    mCerulean
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    CAGGS
    Availability
    Academic Institutions and Nonprofits only
    140759-plasmid-map-sequence-id-311014

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  4. JPF0419d

    Plasmid
    #124020
    Purpose
    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    124020-plasmid-map-sequence-id-262655

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  5. p3E_he1a:ECFP

    Plasmid
    #113880
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Marnie Halpern
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    ECFP
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113880-plasmid-map-sequence-id-222569

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  6. Cerulean(1-158)Stop in pcDNA1/amp

    Plasmid
    #60894
    Purpose
    This vector attaches mCerulean(1-158) to the C-terminus of a protein.
    Depositor
    Catherine Berlot
    Insert
    Cerulean(1-158)Stop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    60894-plasmid-map-sequence-id-112542

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  7. pcDNA3-BG4-NLS-mCer

    Plasmid
    #211448
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Helmut Pospiech
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Use
    Tags
    NLS-mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211448-plasmid-map-sequence-id-419419

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  8. Cer(159-238)-beta-5 in pcDNAI/Amp

    Plasmid
    #55778
    Purpose
    A carboxyl-terminal mCerulean fragment was fused to Gbeta-5. When co-expressed with an amino terminal mCerulean fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    Cer(159-238)-beta-5 (GNB5 Human, Aequorea victoria)
    Use
    Tags
    mCer(159-238) was fused to the amino terminus of ā€¦
    Expression
    Mammalian
    Mutation
    GBeta-5 was amplified via PCR, which added an N-tā€¦
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55778-plasmid-map-sequence-id-114869

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Showing: 281 - 300 of 429 results