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BAS288 C terminal mKO2 natR

Plasmid

#74083

Purpose

S.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

mKO2

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
Omega Destination-CMV::ELuc:bGH

Plasmid

#124528

Purpose

Omega destination vector, that has a CMV:ELUC:bgH in the backbone

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[Spinach2]

Plasmid

#68430

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing the "Spinach2" aptamer

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

Plasmid

#173963

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif

Use

Luciferase

Tags

Expression

Mutation

1 mutated Coordinator motif

Promoter

SV40 promoter

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-StefinA

Plasmid

#182419

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-stefin (CSTA Human)

Use

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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BAS287 C terminal E2C hygBR

Plasmid

#74082

Purpose

S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

E2Crimson

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
BAS292 C terminal SF-GFP kanR

Plasmid

#74087

Purpose

S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

SF-GFP recoded for S.pombe

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
BAS286 C terminal SF-GFP hygBR

Plasmid

#74081

Purpose

S.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistanc

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

super-folder GFP

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK-kinase0

Plasmid

#59122

Purpose

FAK fret control sensor without FERM domain with no linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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IFI16-(192-393)-HinA

Plasmid

#35052

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains HinA domain: aa 192-393

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
BAS289 C terminal E2C G418R

Plasmid

#74084

Purpose

S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, G418 resistance

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

E2Crimson

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK-kinase10

Plasmid

#59123

Purpose

FAK fret control sensor without FERM domain with 10 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Human Phosphomevalonate kinase (PMK)

Plasmid

#52430

Purpose

bacterial expression of human Phosphomevalonate kinase (PMK)

Depositor

Henry Miziorko

Article

Herdendorf et al Biochemistry. 2006 Mar 14;45(10):3235-42

Insert

Phosphomevalonate kinase (PMVK Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
PX395 Roseburia intestinalis Cas9

Plasmid

#68332

Purpose

Roseburia intestinalis Cas9

Depositor

Feng Zhang

Insert

RiCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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BAS291 C terminal mKO2 kanR

Plasmid

#74086

Purpose

S.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 resistance.

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

mKO2

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
BAS290 C terminal E2C natR

Plasmid

#74085

Purpose

S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, nourseothricin resistance

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

E2Crimson

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pET-28b C7H1G6_6H N-terminal

Plasmid

#223434

Purpose

C7H1G6 GNAT expression vector with N-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

C7H1G6 GNAT

Use

Tags

His6

Expression

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b R6CZ24_6H C-terminal

Plasmid

#223435

Purpose

R6CZ24 Thiolase expression vector with C-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

R6CZ24 Thiolase

Use

Tags

His6

Expression

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

Plasmid

#217662

Purpose

expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymCardinal-T2A-mtq2

Plasmid

#205508

Purpose

Expression of ymCardinal-T2A-mtq2 in yeast

Depositor

Bas Teusink

Insert

ymCardinal-T2A-mtq2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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