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pSLQ5079_pHR_PGK_sfGFP_CoV-F1

Plasmid

#155303

Purpose

SARS-CoV-2 fluorescent reporter 1

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Superfolder GFP fused with SARS-CoV-F1 (ORF1ab Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc F-tractinN9-39-msfGFPΔN7

Plasmid

#231552

Purpose

Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

F-tractin (Itpka Rat)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMVtrunc

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-S(4+)-EGFP

Plasmid

#21317

Purpose

Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EOS-S(4+) promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-C(3+)-EGFP

Plasmid

#21318

Purpose

Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EOS-C(3+) promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pPB-NeoCOVGT3-d2EGFP

Plasmid

#201446

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT3_d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-CoV2-WT

Plasmid

#177618

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-PI35P2

Plasmid

#92419

Purpose

Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

Depositor

Geert van den Bogaart

Article

Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

Insert

MCOLN1 (Mcoln1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Synthetic gene, codon optimized for human.

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.GFP.miR-99a

Plasmid

#169304

Purpose

Constitutive overexpression of miR-99a with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-99a (MIR99A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBactin-AcGFP-CI

Plasmid

#196849

Purpose

Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

AcGFP

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.dTomato.miR-ctrl.PGK.sfGFP.P2A.Tet3G

Plasmid

#169316

Purpose

Doxycycline-inducible expression of miR-ctrl with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

TRE

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.GFP.miR-ctrl

Plasmid

#169305

Purpose

Constitutive overexpression of miR-ctrl with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

SFFV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc msfGFPΔC10-beta-actin

Plasmid

#231548

Purpose

Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

ACTB (ACTB Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMVtrunc

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc msfGFPΔC10-Utr28-222

Plasmid

#231558

Purpose

Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Utrophin calponin homology domain (UTRN Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMVtrunc

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa (T286D)

Plasmid

#127391

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 286 to Aspartic acid

Promoter

pCAG

Available since

Jan. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa (T286A)

Plasmid

#127390

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 286 to Alanine

Promoter

pCAG

Available since

Jan. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT1-d2EGFP

Plasmid

#201447

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT1_d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-Neo-COVGT2-d2EGFP

Plasmid

#201448

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT2_d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-Neo-COVGT4-d2EGFP

Plasmid

#201449

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT4_d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-NeoCOVGT3-d2EGFP-mCherry

Plasmid

#201450

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT3_d2EGFP-mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-Inframe-noFSE

Plasmid

#177619

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

None

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge